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Vitamin D Receptor Is Required for Proliferation, Migration, and Differentiation of Epidermal Stem Cells and Progeny during Cutaneous Wound Repair.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2018 Nov; Vol. 138 (11), pp. 2423-2431. Date of Electronic Publication: 2018 May 19. - Publication Year :
- 2018
-
Abstract
- Epidermal stem cells residing in the skin play an essential role in epidermal regeneration. When skin is injured, the stem cells are first activated to proliferate, and subsequently the progeny migrate and differentiate to regenerate the epidermis. Here, we demonstrate that the vitamin D receptor (VDR) is essential for these processes to occur. The requirement for VDR on epidermal stem cell function was revealed in conditional VDR knockout mice, in which VDR was deleted from stem cells and progeny, and mice were maintained on a low calcium diet. First, self-renewal and niche formation of epidermal stem cells were impaired. Wound-induced activation of epidermal stem cells was blunted associated with a reduction of β-catenin signaling. Second, wound induced migration of stem cells and progeny was impaired as shown by lineage tracing and delayed migration of VDR silenced cells. Epidermal differentiation of progeny was impaired at the wounding site associated with reduced E-cadherin expression. Deletion of VDR also changed stem cell fate blunting hair development, increasing sebaceous glands, and altering expression and location of epidermal markers. These results suggest that VDR is required for self-renewal, migration, and differentiation of epidermal stem cells and progeny during cutaneous wound healing.<br /> (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Differentiation
Cell Movement
Cell Proliferation
Cell Self Renewal
Cell Transdifferentiation
Cellular Reprogramming
Mice
Mice, Knockout
Receptors, Calcitriol genetics
Regeneration
Sebaceous Glands
Signal Transduction
Wound Healing
beta Catenin metabolism
Epidermal Cells physiology
Receptors, Calcitriol metabolism
Skin pathology
Stem Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1747
- Volume :
- 138
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 29787748
- Full Text :
- https://doi.org/10.1016/j.jid.2018.04.033