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Long noncoding RNA myocardial infarction‑associated transcript is associated with the microRNA‑150‑5p/P300 pathway in cardiac hypertrophy.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2018 Sep; Vol. 42 (3), pp. 1265-1272. Date of Electronic Publication: 2018 May 21. - Publication Year :
- 2018
-
Abstract
- In numerous diseases, abnormal expression of myocardial infarction‑associated transcript (MIAT) has been reported to be involved in cell proliferation, apoptosis and migration. However, whether this long non‑coding RNA MIAT has a regulatory effect on heart hypertrophy requires further investigation. To this end, the present study evaluated MIAT in hypertrophic cardiomyocytes in vitro and in vivo. Neonatal rat ventricular myocytes (NRVMs) were induced by isoproterenol (ISO) to create a cell hypertrophy model, and mice were intraperitoneally injected with ISO to establish an animal model. Echocardiography, immunofluorescence staining, western blot analysis, RNA isolation and reverse transcription‑polymerase chain reaction were applied to test the involvement of MIAT in cardiac hypertrophy. The results revealed that MIAT was upregulated under ISO stimulation at the mRNA level both in vivo and in vitro. Silencing of MIAT resulted in decreased expression levels of atrial natriuretic peptide and brain natriuretic peptide in ISO‑treated NRVM cardiomyocytes, confirming the connection between MIAT and hypertrophy. Furthermore, MIAT small interfering RNA significantly increased microRNA (miR)‑150 and decreased P300 expression in NRVMs. In conclusion, the MIAT/miR‑150‑5p axis targets P300 as a positive regulator of cardiomyocyte hypertrophy.
- Subjects :
- Animals
Blotting, Western
Cardiomegaly genetics
Echocardiography
Male
Mice
Mice, Inbred C57BL
MicroRNAs genetics
Muscle Cells cytology
Muscle Cells metabolism
Myocardial Infarction genetics
RNA, Long Noncoding genetics
Rats
Cardiomegaly metabolism
MicroRNAs metabolism
Myocardial Infarction metabolism
Myocytes, Cardiac metabolism
RNA, Long Noncoding physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 42
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 29786749
- Full Text :
- https://doi.org/10.3892/ijmm.2018.3700