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Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps.
- Source :
-
The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2018 Aug; Vol. 142 (2), pp. 460-469.e7. Date of Electronic Publication: 2018 May 17. - Publication Year :
- 2018
-
Abstract
- Background: IL-25 can function as an early signal for the respiratory type 2 response characteristic of allergic asthma and chronic rhinosinusitis with nasal polyps (CRSwNP). In the mouse gut, tuft cells are the epithelial source of IL-25. However, the source of human airway epithelial IL-25 has remained elusive.<br />Objective: In this study we sought to determine whether the solitary chemosensory cell (SCC) is the predominant source of IL-25 in the sinonasal epithelium.<br />Method: Flow cytometry and immunofluorescence for SCCs and IL-25 were used to interrogate polyp and turbinate tissue from patients with CRSwNP. Mucus was collected during acute inflammatory exacerbations from patients with CRSwNP or chronic rhinosinusitis without nasal polyps and IL-25 levels determined by using ELISA. Lastly, sinonasal epithelial cultures derived from polyp and turbinate tissue were stimulated with IL-13 and analyzed for SCC proliferation and IL-25 production.<br />Results: This study demonstrates that a discrete cell type, likely an SCC, characterized by expression of the taste-associated G protein gustducin and the intestinal tuft cell marker doublecortin-like kinase 1, is the predominant source of IL-25 in the human upper airway. Additionally, we show that patients with CRSwNP have increased numbers of SCCs in nasal polyp tissue and that in vitro IL-13 exposure both increased proliferation and induced apical secretion of IL-25 into the mucosal layer.<br />Conclusions: Inflammatory sinus polyps but not adjacent turbinate tissue show expansion of the SCC population, which is the source of epithelial IL-25.<br /> (Published by Elsevier Inc.)
- Subjects :
- Animals
Cells, Cultured
Chronic Disease
Doublecortin-Like Kinases
Flow Cytometry
Humans
Interleukin-13 metabolism
Intracellular Signaling Peptides and Proteins metabolism
Mice
Protein Serine-Threonine Kinases metabolism
Taste physiology
Transducin metabolism
Chemoreceptor Cells physiology
Interleukin-17 metabolism
Nasal Polyps immunology
Paranasal Sinuses pathology
Respiratory Mucosa physiology
Rhinitis immunology
Sinusitis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-6825
- Volume :
- 142
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of allergy and clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29778504
- Full Text :
- https://doi.org/10.1016/j.jaci.2018.03.019