Revealing A-Raf functions through its interactome.

Authors :: Zhang XY
Guo H
Han B
Zhang XM
Huang Y
Yang Y
Liu Y
Guo XX
Hao Q
An S
Xu TR
Source :: Biochimica et biophysica acta. Proteins and proteomics [Biochim Biophys Acta Proteins Proteom] 2018 Aug; Vol. 1866 (8), pp. 849-856. Date of Electronic Publication: 2018 May 16.
Publication Year :: 2018
Abstract: A-Raf is a member of the Raf kinase family. Unlike B-Raf and C-Raf, the functions of A-Raf remain obscure. To gain more insight into the biological functions of A-Raf, we investigated the A-Raf interactome using proteomics. We found 132 proteins that interact with A-Raf and confirmed the interaction of 12 of these proteins with A-Raf by western blotting. Our data suggested that A-Raf regulates apoptosis, RNA catabolism, GTPase activity, and cell adhesion by interacting with proteins located in different cellular compartments. We identified all ten hallmarks of cancer in these interacting proteins, suggesting that A-Raf is involved in carcinogenesis. Our results also indicated that A-Raf may play a role in different diseases and signaling pathways. These findings have identified potential regulators of A-Raf and provide a systemic insight into its biological functions.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)