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Analysis of genetic variability of respiratory syncytial virus groups A and B in Kuwait.
- Source :
-
Archives of virology [Arch Virol] 2018 Sep; Vol. 163 (9), pp. 2405-2413. Date of Electronic Publication: 2018 May 19. - Publication Year :
- 2018
-
Abstract
- Respiratory syncytial virus (RSV) is the most frequently identified viral agent in infants, children, and elderly people with acute respiratory tract infections (ARTIs). This study is the only one of its kind in Kuwait, and its purpose was to investigate the genetic variability of the G protein gene in RSV strains prevalent in Kuwait. Respiratory samples were collected from patients with ARTIs in various hospitals in Kuwait and subjected to reverse transcription PCR (RT-PCR) amplifying a fragment of the G gene of RSV. A total of 305 samples were collected between January and mid-December 2016, and 77 (25.2%) were positive for RSV. Group A viruses were predominant over group B viruses; the RSV-A group was detected in 52 (67.5%) of the positive samples, while the RSV-B group was detected in 25 (32.5%) of the positive samples. Phylogenetic analysis showed that all RSV-A strains grouped into eight clusters of identical sequences of untyped strains. Twelve RSV-B strains, on the other hand, belonged to the RSV-B/BA10 genotype, while the rest were untyped. These data suggest that new and untyped strains of RSV-A group likely predominated in Kuwait and that the BA10 genotype of the RSV-B group became the dominant genotype in the 2016 season.
- Subjects :
- Acute Disease
Adolescent
Adult
Aged
Child
Child, Preschool
Humans
Infant
Infant, Newborn
Kuwait epidemiology
Middle Aged
Phylogeny
Prevalence
Respiratory Syncytial Virus Infections pathology
Respiratory Syncytial Virus Infections virology
Respiratory Syncytial Virus, Human classification
Respiratory Syncytial Virus, Human isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Seasons
Sequence Analysis, DNA
Genetic Variation
Genotype
RNA, Viral genetics
Respiratory Syncytial Virus Infections epidemiology
Respiratory Syncytial Virus, Human genetics
Viral Fusion Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 163
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 29777370
- Full Text :
- https://doi.org/10.1007/s00705-018-3881-z