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Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine.
- Source :
-
Scientific reports [Sci Rep] 2018 May 10; Vol. 8 (1), pp. 7481. Date of Electronic Publication: 2018 May 10. - Publication Year :
- 2018
-
Abstract
- Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specific pathogen-free swine. A CHO-heparan sulfate-deficient (CHO-HS(-)) cell clone, named 51-10 clone, stably expressing GI-JEV VLP was selected and continually secreted GI VLPs without signs of cell fusion. 51-10 VLPs formed a homogeneously empty-particle morphology and exhibited similar antigenic activity as GI virus. GI VLP-immunized mice showed balanced cross-neutralizing antibody titers against GI to GIV viruses (50% focus-reduction micro-neutralization assay titers 71 to 240) as well as potent protection against GI or GIII virus infection. GI VLP-immunized swine challenged with GI or GIII viruses showed no fever, viremia, or viral RNA in tonsils, lymph nodes, and brains as compared with phosphate buffered saline-immunized swine. We thus conclude GI VLPs can provide sterile protection against GI and GIII viruses in swine.
- Subjects :
- Animals
Antibodies, Neutralizing genetics
CHO Cells
COS Cells
Chlorocebus aethiops
Cricetinae
Cricetulus
Disease Models, Animal
Encephalitis Virus, Japanese classification
Encephalitis, Japanese genetics
Encephalitis, Japanese immunology
Female
Genotype
Japanese Encephalitis Vaccines genetics
Japanese Encephalitis Vaccines immunology
Mice
Mice, Inbred BALB C
RNA, Viral genetics
Swine
Vaccination veterinary
Vero Cells
Virion genetics
Virion immunology
Antibodies, Neutralizing therapeutic use
Cross Protection genetics
Cross Protection immunology
Encephalitis Virus, Japanese genetics
Encephalitis Virus, Japanese immunology
Encephalitis, Japanese therapy
Japanese Encephalitis Vaccines therapeutic use
Vaccination methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29748549
- Full Text :
- https://doi.org/10.1038/s41598-018-25596-1