A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells.

Authors :: Mehta R
Qureshi MH
Purchal MK
Greer SM
Gong S
Ngo C
Que EL
Source :: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2018 May 24; Vol. 54 (43), pp. 5442-5445.
Publication Year :: 2018
Abstract: We report the synthesis and application of a small molecule probe for carbonic anhydrase (CA) to track holo-CA in cell lysates and live-cell models of zinc dyshomeostasis. The probe displays a 12-fold increase in fluorescence upon binding to bovine CA and also responds to human CA isoforms.

Subjects :: Animals
Carbonic Anhydrases metabolism
Cattle
Erythrocytes cytology
Humans
Molecular Structure
Zinc metabolism
Carbonic Anhydrases analysis
Erythrocytes metabolism
Fluorescent Dyes chemistry
Small Molecule Libraries chemistry
Zinc analysis

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