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Progress in One-pot Bioconversion of Cephalosporin C to 7-Aminocephalosporanic Acid.
- Source :
-
Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2018; Vol. 19 (1), pp. 30-42. - Publication Year :
- 2018
-
Abstract
- Background: Cephalosporins are the most widely used semisynthetic antibiotics, which acted on bacterial cell wall (peptidoglycan) synthesis. The key intermediate for fabricating about twothirds of cephalosporins in clinical use is 7-aminocephalosporanic acid (7-ACA), which is derived from chemical or enzymatic deacylation of the natural antibiotic cephalosporin C (CPC). The chemical deacylation process has been replaced by the enzymatic deacylation process because the chemical process required harsh conditions and released toxic waste.<br />Methods: A two-step enzymatic process that utilized D-amino acid oxidase (DAAO) and 7-β-(4carboxybutanamido)-cephalosporanic acid acylase (GLA) for two successive reactions has been applied for the conversion of CPC to 7-ACA in an industrial scale.<br />Results: To simplify the process and lower costs, the one-pot enzymatic processes were developed by the application of the mono-enzymatic process (application of cephalosporin C acylase or the variants of GLA), di-enzymatic process (simultaneous action of DAAO and GLA) or the tri-enzymatic process (simultaneous action of DAAO, GLA and catalase) for direct conversion of CPC to 7-ACA.<br />Conclusion: Here, we mainly focused on the description of these one-pot enzymatic processes and emphasized on the preparation of the involved biocatalysts.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
Details
- Language :
- English
- ISSN :
- 1873-4316
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Current pharmaceutical biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29745327
- Full Text :
- https://doi.org/10.2174/1389201019666180509093956