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Epigenetic Regulation of Aldosterone Synthase Gene by Sodium and Angiotensin II.
- Source :
-
Journal of the American Heart Association [J Am Heart Assoc] 2018 May 08; Vol. 7 (10). Date of Electronic Publication: 2018 May 08. - Publication Year :
- 2018
-
Abstract
- Background: DNA methylation is believed to be maintained in adult somatic cells. Recent findings, however, suggest that all methylation patterns are not stable. We demonstrate that stimulatory signals can change the DNA methylation status around transcription factor binding sites and a transcription start site and activate expression of the aldosterone synthase gene ( CYP11B2 ).<br />Methods and Results: DNA methylation of CYP11B2 was analyzed in aldosterone-producing adenomas, nonfunctioning adrenal adenomas, and adrenal glands and compared with the gene expression levels. CpG dinucleotides in the CYP11B2 promoter were found to be hypormethylated in tissues with high expression, but not in those with low expression, of CYP11B2 . Methylation of the CYP11B2 promoter fused to a reporter gene decreased transcriptional activity. Methylation of recognition sequences of transcription factors, including CREB1, NGFIB (NR4A1), and NURR1 (NR4A2) diminished their DNA-binding activity. A methylated-CpG-binding protein MECP2 interacted directly with the methylated CYP11B2 promoter. In rats, low salt intake led to upregulation of CYP11B2 expression and DNA hypomethylation in the adrenal gland. Treatment with angiotensin II type 1 receptor antagonist decreased CYP11B2 expression and led to DNA hypermethylation.<br />Conclusions: DNA demethylation may switch the phenotype of CYP11B2 expression from an inactive to an active state and regulate aldosterone biosynthesis.<br /> (© 2018 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.)
- Subjects :
- Adrenal Glands drug effects
Angiotensin II Type 1 Receptor Blockers pharmacology
Animals
Binding Sites
Cell Line, Tumor
CpG Islands
Cytochrome P-450 CYP11B2 metabolism
Diet, Sodium-Restricted
Gene Expression Regulation, Enzymologic
Humans
Male
Promoter Regions, Genetic
Rats, Wistar
Transcription Factors genetics
Transcription Factors metabolism
Transcription, Genetic drug effects
Adrenal Glands enzymology
Aldosterone biosynthesis
Angiotensin II metabolism
Cytochrome P-450 CYP11B2 genetics
DNA Methylation drug effects
Epigenesis, Genetic drug effects
Sodium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2047-9980
- Volume :
- 7
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of the American Heart Association
- Publication Type :
- Academic Journal
- Accession number :
- 29739797
- Full Text :
- https://doi.org/10.1161/JAHA.117.008281