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[Effects of MSP on Cell Cycle and EMT of Non-Small Cell Lung Cancer PC14].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2018 Mar; Vol. 49 (2), pp. 205-208. - Publication Year :
- 2018
-
Abstract
- Objective: To study the effect of macrophage stimulating protein (MSP) on the cell cycle of non-small cell lung cancer PC14 cells without expression of recepteur d'originenanta (RON) and MSP,and analyse its effect on PC14's epithelial mesenchymal transition (EMT) capacity.<br />Methods: Vitro culture PC14 (blank control),PC14-Mst1-pEGFP-N1 (stablely expressed MSP) and PC14-pEGFP-N1. Cell cycles were detected by flow cytometry and the gaps between cells during growth were measured by transmission electron microscope (TEM); RT-PCR and Western blot were used to figure out the shifts of EMT related gene expression in PC14-Mst1-pEGFP-N1 cells.<br />Results: Compared with the PC14 group and PC14-pEGFP-N1 group,PC14-Mst1-pEGFP-N1 population of G1/G0 phase were significantly increased while S and G2/M phase were significantly reduced;The gaps between PC14-Mst1-pEGFP-N1 cells decreased; RT-PCR and Western blot showed that mRNA and protein levels of E-cadherin of PC14-Mst1-pEGFP-N1 were significantly higher than that of PC14,but mRNA and protein levels of Vimentin were significantly lower.<br />Conclusion: MSP may affect the cell cycle of PC14 and inhibit its EMT procedure by regulating the expression of related proteins including E-cadherin and Vimentin when RON was not expressed.<br /> (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)
- Subjects :
- Antigens, CD metabolism
Cadherins metabolism
Cell Line, Tumor
Cell Proliferation
Humans
Intracellular Signaling Peptides and Proteins
Protein Serine-Threonine Kinases genetics
Vimentin metabolism
Carcinoma, Non-Small-Cell Lung genetics
Cell Cycle
Epithelial-Mesenchymal Transition
Hepatocyte Growth Factor genetics
Lung Neoplasms genetics
Proto-Oncogene Proteins genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 29737061