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Vitamin D Supplementation Reverses DNA Damage and Telomeres Shortening Caused by Ovariectomy in Hippocampus of Wistar Rats.
- Source :
-
Neurotoxicity research [Neurotox Res] 2018 Oct; Vol. 34 (3), pp. 538-546. Date of Electronic Publication: 2018 May 05. - Publication Year :
- 2018
-
Abstract
- The aim of this study was to investigate the effect of ovariectomy (OVX), a surgical model of menopause, and/or vitamin D (VIT D) supplementation on oxidative status, DNA damage, and telomere length in hippocampus of rats at two ages. Ninety-day-old (adult) or 180-day-old (older) female Wistar rats were divided into four groups: SHAM, OVX, VIT D, and OVX + VIT D. Thirty days after OVX, rats were supplemented with VIT D (500 IU/kg) by gavage, for a period of 30 days. Results showed that OVX altered antioxidant enzymes, increasing the activities of catalase in adult rats and superoxide dismutase in older rats. VIT D per se increased the activities of catalase and superoxide dismutase in older rats, but not in adult rats. VIT D supplementation to OVX (OVX + VIT D) rats did not reverse the effect of OVX on catalase in adult rats, but it partially reversed the increase in superoxide dismutase activity in older rats. OVX increased DNA damage in hippocampus of adult and older rats. VIT D per se reduced DNA damage, and when associated to OVX, it partially reversed this alteration. Additionally, OVX caused a telomere shortening in older rats, and VIT D was able to reverse such effect. Taken together, these results demonstrate that surgical menopause in rats causes hippocampal biochemical changes and VIT D appears, at least in part, to act in a beneficial way.
- Subjects :
- Age Factors
Animals
Catalase metabolism
Comet Assay
Female
Oxidative Stress drug effects
Rats
Rats, Wistar
Statistics, Nonparametric
Superoxide Dismutase metabolism
Telomere Shortening drug effects
Thiobarbituric Acid Reactive Substances metabolism
Time Factors
DNA Damage drug effects
Hippocampus drug effects
Ovariectomy adverse effects
Telomere Shortening physiology
Vitamin D pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1476-3524
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neurotoxicity research
- Publication Type :
- Academic Journal
- Accession number :
- 29730834
- Full Text :
- https://doi.org/10.1007/s12640-018-9909-z