Back to Search
Start Over
A real-time RT-PCR assay for molecular identification and quantitation of feline morbillivirus RNA from biological specimens.
- Source :
-
Journal of virological methods [J Virol Methods] 2018 Aug; Vol. 258, pp. 24-28. Date of Electronic Publication: 2018 May 03. - Publication Year :
- 2018
-
Abstract
- The aim of this study was to develop a real-time RT-PCR to detect and quantitate feline morbillivirus (FeMV) RNA in biological samples. Primers and probe were targeted on a conserved region of FeMV P/V/C gene. To validate the assay with field samples, a total number of specimens of cats have been recruited including 264 urine and blood samples and compared with a generic RT-PCR targeting the L protein encoding gene of morbilliviruses. In addition, 385 tissue samples from 35 carcasses of cats have been also employed. RNA titres were low in all tested samples. Results also indicated the absence of cross-reaction with related morbilliviruses and existing pathogens of cats. In tissues with low levels of FeMV RNA, the presence of viral antigen was also evidenced by immunohistochemistry targeting the N viral protein. This newly described assay allows for a rapid, accurate and reliable quantitative detection of FeMV RNA that can be applied for diagnostics and research studies.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cats
DNA Primers genetics
Morbillivirus genetics
Morbillivirus Infections diagnosis
Morbillivirus Infections virology
Oligonucleotide Probes genetics
RNA, Viral genetics
Time Factors
Cat Diseases diagnosis
Cat Diseases virology
Molecular Diagnostic Techniques methods
Morbillivirus isolation & purification
Morbillivirus Infections veterinary
Real-Time Polymerase Chain Reaction methods
Reverse Transcriptase Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0984
- Volume :
- 258
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 29730392
- Full Text :
- https://doi.org/10.1016/j.jviromet.2018.05.002