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Statistical optimization of process parameters for inulinase production from Tithonia weed by Arthrobacter mysorens strain no.1.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2018 Jun; Vol. 149, pp. 55-66. Date of Electronic Publication: 2018 May 03. - Publication Year :
- 2018
-
Abstract
- Tithonia rotundifolia is an easily available and abundant inulin rich weed reported to be competitive and allelopathic. This weed inulin is hydrolyzed by inulinase into fructose. Response surface methodology was employed to optimize culture conditions for the inulinase production from Arthrobacter mysorens strain no.1 isolated from rhizospheric area of Tithonia weed. Initially, Plackett- Burman design was used for screening 11 nutritional parameters for inulinase production including inulin containing weeds as cost effective substrate. The experiment shows that amongst the 11 parameters studied, K <subscript>2</subscript> HPO <subscript>4</subscript> , Inulin, Agave sisalana extract and Tithonia rotundifolia were the most significant variables for inulinase production. Quantitative effects of these 4 factors were further investigated using Box Behnken design. The medium having 0.27% K <subscript>2</subscript> HPO <subscript>4</subscript> , 2.54% Inulin, 6.57% Agave sisalana extract and 7.27% Tithonia rotundifolia extract were found to be optimum for maximum inulinase production. The optimization strategies used showed 2.12 fold increase in inulinase yield (1669.45 EU/ml) compared to non-optimized medium (787 EU/ml). Fructose produced by the action of inulinase was further confirmed by spectrophotometer, osazone, HPTLC and FTIR methods. Thus Tithonia rotundifolia can be used as an eco-friendly, economically feasible and promising alternative substrate for commercial inulinase production yielding fructose from Arthrobacter mysorens strain no.1.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Agave chemistry
Analysis of Variance
Arthrobacter classification
Arthrobacter genetics
Arthrobacter isolation & purification
Culture Media chemistry
Culture Media economics
Fermentation
Fructose metabolism
Inulin metabolism
Phylogeny
Plant Extracts chemistry
Plant Extracts economics
RNA, Ribosomal, 16S genetics
Rhizosphere
Arthrobacter metabolism
Asteraceae chemistry
Asteraceae microbiology
Glycoside Hydrolases biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 149
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 29730324
- Full Text :
- https://doi.org/10.1016/j.mimet.2018.04.019