Back to Search
Start Over
Psychoneuroimmunology and Natural Killer Cells: The Chromium-Release Whole-Blood Assay.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1781, pp. 209-220. - Publication Year :
- 2018
-
Abstract
- Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter describes a chromium ( <superscript>51</superscript> Cr)-release bioassay designed to measure to the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count. Effector cells are defined as CD3-CD56+ lymphocytes. Target cells are the K562 erythroleukemia cell line. Killing capacity is defined as number of target cells killed per effector cell, at an effector cell/target cell ratio of 1:1 during a 4-h in vitro assay.
- Subjects :
- Biological Assay
CD56 Antigen immunology
Case-Control Studies
Chromium immunology
Fatigue Syndrome, Chronic blood
Fatigue Syndrome, Chronic pathology
Flow Cytometry
Humans
K562 Cells
Killer Cells, Natural cytology
Persian Gulf Syndrome blood
Persian Gulf Syndrome pathology
Chromium blood
Cytotoxicity Tests, Immunologic methods
Fatigue Syndrome, Chronic immunology
Killer Cells, Natural immunology
Persian Gulf Syndrome immunology
Psychoneuroimmunology methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1781
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 29705850
- Full Text :
- https://doi.org/10.1007/978-1-4939-7828-1_12