Back to Search
Start Over
Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans.
- Source :
-
Pathogens and disease [Pathog Dis] 2018 Jun 01; Vol. 76 (4). - Publication Year :
- 2018
-
Abstract
- Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.
- Subjects :
- A549 Cells
Animals
Biofilms growth & development
Cell Survival drug effects
Embryo, Nonmammalian
Gene Expression
Hemolysin Proteins biosynthesis
Hemolysin Proteins genetics
Hemolysis drug effects
Host Specificity
Humans
Machine Learning
Pseudomonas Infections microbiology
Pseudomonas Infections pathology
Pseudomonas aeruginosa growth & development
Pseudomonas aeruginosa metabolism
Pyocyanine biosynthesis
Pyocyanine genetics
Virulence
Virulence Factors biosynthesis
Virulence Factors genetics
Zebrafish
Biofilms drug effects
Hemolysin Proteins toxicity
Pseudomonas aeruginosa pathogenicity
Pyocyanine toxicity
Virulence Factors toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 2049-632X
- Volume :
- 76
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Pathogens and disease
- Publication Type :
- Academic Journal
- Accession number :
- 29684116
- Full Text :
- https://doi.org/10.1093/femspd/fty041