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Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [ 18 F]Fluoro-PEG-Folate.
- Source :
-
Contrast media & molecular imaging [Contrast Media Mol Imaging] 2018 Feb 21; Vol. 2018, pp. 8092781. Date of Electronic Publication: 2018 Feb 21 (Print Publication: 2018). - Publication Year :
- 2018
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Abstract
- Background: In rheumatoid arthritis, articular inflammation is a hallmark of disease, while the involvement of extra-articular tissues is less well defined. Here, we examined the feasibility of PET imaging with the macrophage tracer [ <superscript>18</superscript> F]fluoro-PEG-folate, targeting folate receptor β (FR β ), to monitor systemic inflammatory disease in liver and spleen of arthritic rats before and after methotrexate (MTX) treatment.<br />Methods: [ <superscript>18</superscript> F]Fluoro-PEG-folate PET scans (60 min) were acquired in saline- and MTX-treated (1 mg/kg, 4x) arthritic rats, followed by tissue resection and radiotracer distribution analysis. Liver and spleen tissues were stained for ED1/ED2-macrophage markers and FR β expression.<br />Results: [ <superscript>18</superscript> F]Fluoro-PEG-folate PET and ex vivo tissue distribution studies revealed a significant ( p < 0.01) 2-fold lower tracer uptake in both liver and spleen of MTX-treated arthritic rats. Consistently, ED1- and ED2-positive macrophages were significantly ( p < 0.01) decreased in liver (4-fold) and spleen (3-fold) of MTX-treated compared with saline-treated rats. Additionally, FR β -positive macrophages were also significantly reduced in liver (5-fold, p < 0.005) and spleen (3-fold, p < 0.01) of MTX- versus saline-treated rats.<br />Conclusions: MTX treatment reduced activated macrophages in liver and spleen, as markers for systemic inflammation in these organs. Macrophage PET imaging with [ <superscript>18</superscript> F]fluoro-PEG-folate holds promise for detection of systemic inflammation in RA as well as therapy (MTX) response monitoring.
- Subjects :
- Animals
Folic Acid pharmacology
Inflammation chemically induced
Inflammation diagnostic imaging
Inflammation metabolism
Macrophages metabolism
Macrophages pathology
Methotrexate pharmacokinetics
Rats
Rats, Wistar
Arthritis, Experimental chemically induced
Arthritis, Experimental diagnostic imaging
Arthritis, Experimental metabolism
Arthritis, Rheumatoid chemically induced
Arthritis, Rheumatoid diagnostic imaging
Arthritis, Rheumatoid metabolism
Fluorine Radioisotopes pharmacology
Folic Acid analogs & derivatives
Methotrexate pharmacology
Polyethylene Glycols pharmacology
Positron-Emission Tomography
Subjects
Details
- Language :
- English
- ISSN :
- 1555-4317
- Volume :
- 2018
- Database :
- MEDLINE
- Journal :
- Contrast media & molecular imaging
- Publication Type :
- Academic Journal
- Accession number :
- 29681783
- Full Text :
- https://doi.org/10.1155/2018/8092781