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Serum retinol binding protein 4 and galectin-3 binding protein as novel markers for postmenopausal nonalcoholic fatty liver disease.
- Source :
-
Clinical biochemistry [Clin Biochem] 2018 Jun; Vol. 56, pp. 95-101. Date of Electronic Publication: 2018 Apr 19. - Publication Year :
- 2018
-
Abstract
- Objective: To investigate the differential protein expression before and after menopause in women with nonalcoholic fatty liver disease (NAFLD) and to explore novel markers for menopausal NAFLD.<br />Methods: Eight serum samples collected from pre- or post-menopausal women with NAFLD were analysed by iTRAQ 2D-LC-MS/MS. Two protein candidates were selected and verified by enzyme-linked immunosorbent assay (ELISA) in serum samples collected from a one hundred and fifty-three female population subsequently, including 51 in post-menopausal status with NAFLD, 41 in pre-menopausal with NAFLD, 19 healthy individuals in post-menopausal status and 42 healthy pre-menopausal women.<br />Results: A total of one hundred and sixty-seven proteins exhibiting significant changes were characterized, among which sixty-five were up-regulated and one hundred and two were down-regulated. Of those altered proteins, the expression of serum retinol binding protein 4 (RBP4) and galectin-3 binding protein (LGALS3BP) was obviously increased in the post-menopausal patient group compared to the other. ELISA validations for the two proteins were consistent with the proteomic profiling.<br />Conclusions: Serum RBP4 and LGAL3BP were up-regulated after menopause under NAFLD conditions, which suggested the two proteins may be potential markers as NAFLD in postmenopausal population.<br /> (Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Antigens, Neoplasm chemistry
Antigens, Neoplasm metabolism
Biomarkers blood
Biomarkers, Tumor chemistry
Biomarkers, Tumor metabolism
Carrier Proteins chemistry
Carrier Proteins metabolism
China
Chromatography, High Pressure Liquid
Cohort Studies
Enzyme-Linked Immunosorbent Assay
Female
Glycoproteins chemistry
Glycoproteins metabolism
Humans
Liver diagnostic imaging
Middle Aged
Non-alcoholic Fatty Liver Disease diagnostic imaging
Non-alcoholic Fatty Liver Disease ethnology
Peptide Mapping
Postmenopause ethnology
Proteomics methods
Reproducibility of Results
Retinol-Binding Proteins, Plasma chemistry
Retinol-Binding Proteins, Plasma metabolism
Tandem Mass Spectrometry
Ultrasonography
Antigens, Neoplasm blood
Biomarkers, Tumor blood
Carrier Proteins blood
Glycoproteins blood
Non-alcoholic Fatty Liver Disease blood
Retinol-Binding Proteins, Plasma analysis
Up-Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2933
- Volume :
- 56
- Database :
- MEDLINE
- Journal :
- Clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 29679552
- Full Text :
- https://doi.org/10.1016/j.clinbiochem.2018.04.017