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The Tomato Mitogen-Activated Protein Kinase SlMPK1 Is as a Negative Regulator of the High-Temperature Stress Response.
- Source :
-
Plant physiology [Plant Physiol] 2018 Jun; Vol. 177 (2), pp. 633-651. Date of Electronic Publication: 2018 Apr 20. - Publication Year :
- 2018
-
Abstract
- High-temperature (HT) stress is a major environmental stress that limits plant growth and development. MAPK cascades play key roles in plant growth and stress signaling, but their involvement in the HT stress response is poorly understood. Here, we describe a 47-kD MBP-phosphorylated protein (p47-MBPK) activated in tomato ( Solanum lycopersicum ) leaves under HT and identify it as SlMPK1 by tandem mass spectrometry analysis. Silencing of SlMPK1 in transgenic tomato plants resulted in enhanced tolerance to HT, while overexpression resulted in reduced tolerance. Proteomic analysis identified a set of proteins involved in antioxidant defense that are significantly more abundant in RNA interference- SlMPK1 plants than nontransgenic plants under HT stress. RNA interference- SlMPK1 plants also showed changes in membrane lipid peroxidation and antioxidant enzyme activities. Furthermore, using yeast two-hybrid screening, we identified a serine-proline-rich protein homolog, SlSPRH1, which interacts with SlMPK1 in yeast, in plant cells, and in vitro. We demonstrate that SlMPK1 can directly phosphorylate SlSPRH1. Furthermore, the serine residue serine-44 of SlSPRH1 is a crucial phosphorylation site in the SlMPK1-mediated antioxidant defense mechanism activated during HT stress. We also demonstrate that heterologous expression of SlSPRH1 in Arabidopsis ( Arabidopsis thaliana ) led to a decrease in thermotolerance and lower antioxidant capacity. Taken together, our results suggest that SlMPK1 is a negative regulator of thermotolerance in tomato plants. SlMPK1 acts by regulating antioxidant defense, and its substrate SlSPRH1 is involved in this pathway.<br /> (© 2018 American Society of Plant Biologists. All rights reserved.)
- Subjects :
- Antioxidants metabolism
Gene Expression Regulation, Plant
Mitogen-Activated Protein Kinases genetics
Phosphorylation
Plant Leaves metabolism
Plant Proteins genetics
Plants, Genetically Modified
Protein Interaction Mapping
Serine metabolism
Temperature
Heat-Shock Response physiology
Solanum lycopersicum physiology
Mitogen-Activated Protein Kinases metabolism
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 177
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 29678861
- Full Text :
- https://doi.org/10.1104/pp.18.00067