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Upregulation of LncDQ is Associated with Poor Prognosis and Promotes Tumor Progression via Epigenetic Regulation of the EMT Pathway in HCC.
- Source :
-
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2018; Vol. 46 (3), pp. 1122-1133. Date of Electronic Publication: 2018 Apr 13. - Publication Year :
- 2018
-
Abstract
- Background/aims: Long noncoding RNAs (lncRNAs) are key regulators of cancer initiation and progression. In this study, we investigated the clinical value and functional role of LncRNA DQ786243 (LncDQ) in the pathogenesis of hepatocellular carcinoma (HCC).<br />Methods: To investigate the expression level of LncDQ in HCC, we performed quantitative real-time PCR using total RNA extracted from HCC tumor tissues and their matched non-neoplastic counterparts, as well as from the serum of HCC patients and healthy volunteers. The correlation of LncDQ expression with clinicopathologic features and prognosis was analyzed. The functional role of LncDQ in cell proliferation, migration, and invasion were evaluated by MTT cell viability, wound healing, and transwell assays in vitro and in vivo. RNA immunoprecipitation and chromatin immunoprecipitation assays were performed to analyze the potential mechanism of LncDQ in HCC cells.<br />Results: LncDQ was upregulated in both HCC tissue samples and serum and was correlated with low survival rate and adverse clinical pathological characteristics. Multivariate analysis demonstrated that LncDQ expression was an independent prognostic factor for HCC. The area under the receiver operating characteristic curve was 0.804 with a sensitivity of 0.72 and a specificity of 0.8. Knockdown of LncDQ induced inhibition of cell proliferation, migration, and invasion in vitro and in vivo. Mechanistically, LncDQ regulated the epithelial-mesenchymal transition pathway by interacting with EZH2, to epigenetically repress the expression of E-cadherin in HCC cells.<br />Conclusions: Taken together, the results of our study indicate that LncDQ plays a critical role in HCC progression, and may serve as a potential diagnostic and prognostic biomarker for HCC.<br /> (© 2018 The Author(s). Published by S. Karger AG, Basel.)
- Subjects :
- Animals
Carcinoma, Hepatocellular diagnosis
Carcinoma, Hepatocellular metabolism
Carcinoma, Hepatocellular mortality
Cell Line, Tumor
Disease Progression
Enhancer of Zeste Homolog 2 Protein chemistry
Enhancer of Zeste Homolog 2 Protein genetics
Enhancer of Zeste Homolog 2 Protein metabolism
Epithelial-Mesenchymal Transition
Female
Hep G2 Cells
Humans
Liver Neoplasms diagnosis
Liver Neoplasms metabolism
Liver Neoplasms mortality
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
RNA, Long Noncoding antagonists & inhibitors
RNA, Long Noncoding blood
RNA, Long Noncoding genetics
Signal Transduction
Transplantation, Heterologous
Up-Regulation
Carcinoma, Hepatocellular pathology
Epigenesis, Genetic
Liver Neoplasms pathology
RNA, Long Noncoding metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1421-9778
- Volume :
- 46
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 29669339
- Full Text :
- https://doi.org/10.1159/000488841