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The Ajuba family protein Wtip regulates actomyosin contractility during vertebrate neural tube closure.
- Source :
-
Journal of cell science [J Cell Sci] 2018 May 16; Vol. 131 (10). Date of Electronic Publication: 2018 May 16. - Publication Year :
- 2018
-
Abstract
- Ajuba family proteins are implicated in the assembly of cell junctions and have been reported to antagonize Hippo signaling in response to cytoskeletal tension. To assess the role of these proteins in actomyosin contractility, we examined the localization and function of Wtip, a member of the Ajuba family, in Xenopus early embryos. Targeted in vivo depletion of Wtip inhibited apical constriction in neuroepithelial cells and elicited neural tube defects. Fluorescent protein-tagged Wtip showed predominant punctate localization along the cell junctions in the epidermis and a linear junctional pattern in the neuroectoderm. In cells undergoing Shroom3-induced apical constriction, the punctate distribution was reorganized into a linear pattern. Conversely, the linear junctional pattern of Wtip in neuroectoderm changed to a more punctate distribution in cells with reduced myosin II activity. The C-terminal fragment of Wtip physically associated with Shroom3 and interfered with Shroom3 activity and neural fold formation. We therefore propose that Wtip is a tension-sensitive cytoskeletal adaptor that regulates apical constriction during vertebrate neurulation.This article has an associated First Person interview with the first author of the paper.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2018. Published by The Company of Biologists Ltd.)
- Subjects :
- Actins genetics
Actins metabolism
Actomyosin genetics
Adaptor Proteins, Signal Transducing chemistry
Adaptor Proteins, Signal Transducing genetics
Animals
Humans
Microfilament Proteins genetics
Microfilament Proteins metabolism
Multigene Family
Neural Tube metabolism
Protein Binding
Protein Domains
Transcription Factors chemistry
Transcription Factors genetics
Xenopus genetics
Xenopus Proteins chemistry
Xenopus Proteins genetics
Actomyosin physiology
Adaptor Proteins, Signal Transducing metabolism
Neural Tube growth & development
Transcription Factors metabolism
Xenopus growth & development
Xenopus metabolism
Xenopus Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9137
- Volume :
- 131
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 29661847
- Full Text :
- https://doi.org/10.1242/jcs.213884