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Gliadin Peptide Facilitates FITC Dextran Transport across the Non Everted Gut Sac of Rat Small Intestine.
- Source :
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Scientia pharmaceutica [Sci Pharm] 2018 Apr 10; Vol. 86 (2). Date of Electronic Publication: 2018 Apr 10. - Publication Year :
- 2018
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Abstract
- Superoxide dismutase (SOD) is an antioxidant protein. When administered orally, it has low bioavailability due to its low permeation. In a previous study we fused gliadin peptide P51 (LGQQQPFPPQQPYPQPQPF) and gliadin peptide P61 (QQPYPQPQPF) with SOD Citrus limon (SOD&#95;Cl), namely GliSOD&#95;P51 and GliSOD&#95;P61 to increase permeation of SOD&#95;Cl through intestine. In this work, the permeation of fluorescein isothiocyanate (FITC)-Dextran 10 kDa, FD10 and 40 kDa, FD40 as paracellular transport markers across excised rat intestinal wall was investigated with the presence of GliSOD&#95;P51 and GliSOD&#95;P61. A permeability study was performed using non-everted rat intestine by incubating FD10 or FD40 with SOD&#95;Cl, and GliSOD&#95;P61. The presence of SOD&#95;Cl, GliSOD&#95;P51 or GliSOD&#95;P61 inside intestine (apical) and outside intestine (basolateral) was analyzed by protein electrophoresis. The concentration of FD that penetrated to the basolateral solution was analyzed by spectrofluorometry. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed the presence of GliSOD&#95;P51 and GliSOD&#95;P61 but not SOD&#95;Cl in basolateral compartment. The percentage of FD10 but not FD40 and SOD&#95;Cl that penetrated to the basolateral solution significantly increased with the presence of gliadin in GliSOD&#95;P51 and GliSOD&#95;P61. GliSOD&#95;P51 and GliSOD&#95;P61 are able to penetrate the rat intestinal epithelial membrane and the gliadin peptides facilitate FD10 to penetrate the epithelial.<br />Competing Interests: Authors certify that there is no actual or potential conflict of interest in relation to this article.
Details
- Language :
- English
- ISSN :
- 2218-0532
- Volume :
- 86
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Scientia pharmaceutica
- Publication Type :
- Academic Journal
- Accession number :
- 29642636
- Full Text :
- https://doi.org/10.3390/scipharm86020013