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Identification of a new variant of Chlamydia trachomatis in Mexico.
- Source :
-
Enfermedades infecciosas y microbiologia clinica (English ed.) [Enferm Infecc Microbiol Clin (Engl Ed)] 2019 Feb; Vol. 37 (2), pp. 93-99. Date of Electronic Publication: 2018 Apr 07. - Publication Year :
- 2019
-
Abstract
- Introduction: Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico.<br />Methods: Swedish-nvCT was searched in 69C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH).<br />Results: The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98%), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters.<br />Conclusions: Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.<br /> (Copyright © 2018 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.)
- Subjects :
- Bacterial Outer Membrane Proteins genetics
Bacterial Proteins genetics
Base Sequence
Chlamydia Infections epidemiology
Chlamydia trachomatis genetics
Chlamydia trachomatis isolation & purification
Computer Simulation
Female
Genotype
Humans
Infertility, Female epidemiology
Infertility, Female microbiology
Integrases genetics
Mexico epidemiology
Phylogeny
Plasmids genetics
Polymerase Chain Reaction
Sequence Alignment
Sequence Deletion
Sequence Homology, Nucleic Acid
Serogroup
Uterine Cervicitis epidemiology
Uterine Cervicitis microbiology
Chlamydia Infections microbiology
Chlamydia trachomatis classification
DNA, Bacterial genetics
Open Reading Frames genetics
Subjects
Details
- Language :
- English; Spanish; Castilian
- ISSN :
- 2529-993X
- Volume :
- 37
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Enfermedades infecciosas y microbiologia clinica (English ed.)
- Publication Type :
- Academic Journal
- Accession number :
- 29636285
- Full Text :
- https://doi.org/10.1016/j.eimc.2018.02.008