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LemA and Erp Y-like recombinant proteins from Leptospira interrogans protect hamsters from challenge using AddaVax™ as adjuvant.
- Source :
-
Vaccine [Vaccine] 2018 May 03; Vol. 36 (19), pp. 2574-2580. Date of Electronic Publication: 2018 Apr 03. - Publication Year :
- 2018
-
Abstract
- Background: Recombinant subunit vaccines have been extensively evaluated as promising alternatives against leptospirosis. Here, we evaluated two proteins in formulations containing the adjuvant AddaVax™ as vaccine candidates for prevention and control of leptospirosis.<br />Methods: Recombinant proteins rErp Y-like and rLemA were characterized by ELISA to assess their ability to bind extracellular matrix (ECM) components and fibrinogen. Groups of eight hamsters were immunized intramuscularly with rErp Y-like or rLemA mixed with a squalene-based adjuvant (AddaVax), and then vaccine efficacy was determined in terms of protection against a lethal challenge. The humoral immune response was determined by ELISA, and the evidence of sub-lethal infection was evaluated by histopathology and kidney culture.<br />Results: rLemA protein binds laminin, fibrinogen, and collagen type IV, while rErp Y-like interacts with fibrinogen. Significant protection was achieved for rLemA and rErp Y-like vaccines, which showed 87.5% and 62.5% survivals, respectively. On day 28, the humoral immune response was significantly greater in the vaccine groups as compared to that in the control group, and the response was predominantly based on IgG2/3. The surviving animals showed negative results in culture isolation but presented with tissue lesions in the lungs and kidneys.<br />Conclusion: Cumulatively, our findings suggest that LemA and Erp Y-like proteins act as adhesins and are able to protect against mortality, but not against tissue lesions. Moreover, AddaVax is a novel adjuvant with potential for improving the immunogenicity of leptospiral vaccines.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacterial Proteins genetics
Bacterial Vaccines genetics
Bacterial Vaccines immunology
Cricetinae
Female
Leptospira interrogans pathogenicity
Leptospirosis immunology
Male
Mice, Inbred BALB C
Recombinant Proteins immunology
Recombinant Proteins pharmacology
Transcription Factors genetics
Vaccines, Synthetic immunology
Vaccines, Synthetic pharmacology
Adjuvants, Immunologic pharmacology
Bacterial Vaccines pharmacology
Leptospira interrogans genetics
Leptospirosis prevention & control
Polysorbates pharmacology
Squalene pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 36
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 29625765
- Full Text :
- https://doi.org/10.1016/j.vaccine.2018.03.078