Back to Search
Start Over
The Role of 2% Rebamipide Eye Drops Related to Conjunctival Differentiation in Superoxide Dismutase-1 (Sod1) Knockout Mice.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2018 Mar 01; Vol. 59 (3), pp. 1675-1681. - Publication Year :
- 2018
-
Abstract
- Purpose: The superoxide dismutase-1 knockout (Sod1-/-) mouse is an age-related dry eye mouse model. We evaluated the role of 2% rebamipide ophthalmic solution on the conjunctiva and ocular surface alterations in Sod1-/- mice.<br />Methods: Rebamipide eye drops (2%) were instilled in six 50-week-old male Sod1-/- mice and six C57BL/6 strain wild-type (WT) male mice four times a day for 2 weeks. Aqueous tear secretion quantity and tear film breakup time measurements as well as vital stainings were performed. Immunohistochemistry staining of the conjunctiva was performed using SAM pointed domain-containing ETS transcription factor (SPDEF), transglutaminase-1, and involucrin antibodies. Quantitative RT-PCR was carried out to study mRNA expression of the same markers.<br />Results: The mean tear quantities showed no significant changes in both mice strains after treatment (P = 0.24). The mean tear film breakup time (P = 0.003) and vital staining scores significantly improved in the Sod1-/- mice after treatment. Treatment with 2% rebamipide eye drops significantly decreased the corneal fluorescein (P = 0.0093) and Rose Bengal (P = 0.002) staining scores in the Sod1-/- mice. We showed a notable increase in SPDEF and a marked decrease in transglutaminase-1 and involucrin immunohistochemistry stainings, together with a significant increase in SPDEF (P = 0.0003) and a significant decline in transglutaminase-1 (P = 0.0072) and involucrin (P = 0.009) mRNA expression after treatment in the Sod1-/- mice.<br />Conclusions: Topical use of 2% rebamipide drops was observed to improve conjunctival epithelial differentiation and suppress keratinization in the Sod1-/- mice.
- Subjects :
- Alanine pharmacology
Animals
Biomarkers metabolism
Conjunctiva metabolism
Conjunctiva pathology
Disease Models, Animal
Immunohistochemistry
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
RNA, Messenger metabolism
Tears metabolism
Alanine analogs & derivatives
Antioxidants pharmacology
Cell Differentiation drug effects
Conjunctiva drug effects
Dry Eye Syndromes drug therapy
Ophthalmic Solutions pharmacology
Quinolones pharmacology
Superoxide Dismutase-1 deficiency
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 59
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 29625494
- Full Text :
- https://doi.org/10.1167/iovs.17-23213