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Chemical composition and in vitro antibacterial and antiproliferative activities of the essential oil from the leaves of Psidium myrtoides O. Berg (Myrtaceae).
- Source :
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Natural product research [Nat Prod Res] 2019 Sep; Vol. 33 (17), pp. 2566-2570. Date of Electronic Publication: 2018 Apr 03. - Publication Year :
- 2019
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Abstract
- In this study, the chemical composition and antibacterial and antiproliferative potential of the essential oil obtained from fresh leaves of Psidium myrtoides (PM-EO) against oral pathogens and human tumour cell lines were investigated for the first time. GC-FID and GC-MS analyses showed that trans -β-caryophyllene (30.9%), α-humulene (15.9%), α-copaene (7.8%), caryophyllene oxide (7.3%) and α-bisabolol (5.3%) are the major constituents of PM-EO. The antibacterial activity of PM-EO against a panel of oral pathogens was investigated in terms of their minimal inhibitory concentrations (MIC) using the broth microdilution method. PM-EO displayed moderate activity against Streptococcus mitis (MIC = 100 μg/mL), S. sanguinis (MIC = 100 μg/mL), S. sobrinus (MIC = 250 μg/mL), and S. salivarius (MIC = 250 μg/mL), and strong activity against S. mutans (MIC = 62.5 μg/mL). The antiproliferative activity in normal (GM07492A, lung fibroblasts) and tumour cell lines (MCF-7, HeLa, and M059 J) was performed using the XTT assay. PM-EO showed 50% inhibition of normal cell growth at 359.8 ± 6.3 μg/mL. Antiproliferative activity was observed against human tumour cell lines, with IC <subscript>50</subscript> values significantly lower than that obtained for the normal cell line, demonstrating IC <subscript>50</subscript> values for MCF-7 cells (254.5 ± 1.6 μg/mL), HeLa cells (324.2 ± 41.4 μg/mL) and M059 J cells (289.3 ± 10.9 μg/mL). Therefore, the cytotoxicity of PM-EO had little influence on the antibacterial effect, since it showed antibacterial activity at lower concentrations. Our results suggest that PM-EO is a promising source of new antibacterial and antitumour agents.
- Subjects :
- Cell Line, Tumor
Gas Chromatography-Mass Spectrometry
Humans
Microbial Sensitivity Tests
Monocyclic Sesquiterpenes
Myrtaceae chemistry
Plant Extracts pharmacology
Plant Leaves chemistry
Polycyclic Sesquiterpenes
Sesquiterpenes analysis
Anti-Bacterial Agents pharmacology
Antineoplastic Agents pharmacology
Oils, Volatile chemistry
Psidium chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1478-6427
- Volume :
- 33
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Natural product research
- Publication Type :
- Academic Journal
- Accession number :
- 29611435
- Full Text :
- https://doi.org/10.1080/14786419.2018.1457664