Rapid identification of carbapenemase-type bla GES and ESBL-type bla GES using multiplex PCR.

Authors :: Kayama S
Yano R
Yamasaki K
Fukuda C
Nishimura K
Miyamoto H
Ohge H
Sugai M
Source :: Journal of microbiological methods [J Microbiol Methods] 2018 May; Vol. 148, pp. 117-119. Date of Electronic Publication: 2018 Mar 30.
Publication Year :: 2018
Abstract: Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170 N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)

Subjects :: Gram-Negative Bacteria isolation & purification
Gram-Negative Bacterial Infections microbiology
Humans
Japan
Time Factors
beta-Lactamases analysis
beta-Lactamases classification
Bacteriological Techniques methods
Gram-Negative Bacteria enzymology
Multiplex Polymerase Chain Reaction methods
beta-Lactamases genetics

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