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Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats.
- Source :
-
Molecular nutrition & food research [Mol Nutr Food Res] 2018 May; Vol. 62 (9), pp. e1701007. Date of Electronic Publication: 2018 Apr 20. - Publication Year :
- 2018
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Abstract
- Scope: We investigated the interaction between streptozotocin (STZ)-induced diabetes and dietary n6/n3 ratio, and its influence on lipogenesis.<br />Methods and Results: The animals were treated with STZ and fed with different dietary n6/n3 ratios: 1, 7, and 60, or supplemented with DHA/EPA. Gene expression was assessed by RT-PCR and protein expression by western blotting and immunohistochemistry. Fatty acid profile was determined by GC-MS. Pancreas and liver histology were assessed by hematoxylin and eosin (H&E) staining. STZ-induced characteristic changes in all STZ treated groups, including: increased blood glucose, decreased body mass, increased lipid peroxidation and CD36 expression, decreased 16:1n7 and 18:1n7, increases in 20:3n6, decreases in phospholipid (PL) content of 20:4n6, as well as decreases in the expression of SREBP1c, Δ-9-desaturase (Δ9D), and Δ-5-desaturase (Δ5D). Additionally, other changes occurred that were dependent on the n6/n3 ratio. Among the diabetic groups, the lower n6/n3 ratio caused higher lipid peroxidation and CD36 expression, a greater decrease in 20:4n6 and decreased Δ6-desaturase (Δ6D) expression, while the higher n6/n3 ratio caused increased partitioning of 20:4n6 into hepatic neutral lipids (NL), a decrease in 20:5n3 content, and increased β-oxidation.<br />Conclusion: Presented data suggest that the n6/n3 ratio could significantly influence lipogenesis, lipid peroxidation, and β-oxidation in STZ-induced diabetes, which could have clinical significance.<br /> (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Animals
Brain pathology
Delta-5 Fatty Acid Desaturase
Diabetes Mellitus, Type 1 blood
Diabetes Mellitus, Type 1 pathology
Dietary Supplements
Docosahexaenoic Acids administration & dosage
Docosahexaenoic Acids blood
Docosahexaenoic Acids metabolism
Eicosapentaenoic Acid administration & dosage
Eicosapentaenoic Acid blood
Eicosapentaenoic Acid metabolism
Fatty Acid Desaturases genetics
Fatty Acids, Omega-3 blood
Fatty Acids, Omega-3 metabolism
Fatty Acids, Omega-6 blood
Fatty Acids, Omega-6 metabolism
Linoleoyl-CoA Desaturase genetics
Linoleoyl-CoA Desaturase metabolism
Lipid Peroxidation
Liver metabolism
Liver pathology
Male
Neurons metabolism
Neurons pathology
Pancreas metabolism
Pancreas pathology
Random Allocation
Rats, Wistar
Stearoyl-CoA Desaturase genetics
Stearoyl-CoA Desaturase metabolism
Streptozocin
Brain metabolism
Diabetes Mellitus, Type 1 metabolism
Fatty Acid Desaturases metabolism
Fatty Acids, Omega-3 administration & dosage
Fatty Acids, Omega-6 administration & dosage
Gene Expression Regulation, Enzymologic
Liver enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1613-4133
- Volume :
- 62
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular nutrition & food research
- Publication Type :
- Academic Journal
- Accession number :
- 29579359
- Full Text :
- https://doi.org/10.1002/mnfr.201701007