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Clock-dependent chromatin topology modulates circadian transcription and behavior.
- Source :
-
Genes & development [Genes Dev] 2018 Mar 01; Vol. 32 (5-6), pp. 347-358. Date of Electronic Publication: 2018 Mar 23. - Publication Year :
- 2018
-
Abstract
- The circadian clock in animals orchestrates widespread oscillatory gene expression programs, which underlie 24-h rhythms in behavior and physiology. Several studies have shown the possible roles of transcription factors and chromatin marks in controlling cyclic gene expression. However, how daily active enhancers modulate rhythmic gene transcription in mammalian tissues is not known. Using circular chromosome conformation capture (4C) combined with sequencing (4C-seq), we discovered oscillatory promoter-enhancer interactions along the 24-h cycle in the mouse liver and kidney. Rhythms in chromatin interactions were abolished in arrhythmic Bmal1 knockout mice. Deleting a contacted intronic enhancer element in the Cryptochrome 1 ( Cry1 ) gene was sufficient to compromise the rhythmic chromatin contacts in tissues. Moreover, the deletion reduced the daily dynamics of Cry1 transcriptional burst frequency and, remarkably, shortened the circadian period of locomotor activity rhythms. Our results establish oscillating and clock-controlled promoter-enhancer looping as a regulatory layer underlying circadian transcription and behavior.<br /> (© 2018 Mermet et al.; Published by Cold Spring Harbor Laboratory Press.)
- Subjects :
- Animals
CLOCK Proteins genetics
Chromatin genetics
Cryptochromes metabolism
Enhancer Elements, Genetic genetics
Kidney physiology
Liver physiology
Mice
Mice, Knockout
Promoter Regions, Genetic physiology
Sequence Deletion genetics
Chromatin metabolism
Circadian Rhythm genetics
Cryptochromes genetics
Transcription, Genetic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1549-5477
- Volume :
- 32
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Genes & development
- Publication Type :
- Academic Journal
- Accession number :
- 29572261
- Full Text :
- https://doi.org/10.1101/gad.312397.118