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In silico design of Mycobacterium tuberculosis epitope ensemble vaccines.
- Source :
-
Molecular immunology [Mol Immunol] 2018 May; Vol. 97, pp. 56-62. Date of Electronic Publication: 2018 Mar 19. - Publication Year :
- 2018
-
Abstract
- Effective control of Mycobacterium tuberculosis is a global necessity. In 2015, tuberculosis (TB) caused more deaths than HIV. Considering the increasing prevalence of multi-drug resistant forms of M. tuberculosis, the need for effective TB vaccines becomes imperative. Currently, the only licensed TB vaccine is Bacillus Calmette-Guérin (BCG). Yet, BCG has many drawbacks limiting its efficacy and applicability. We applied advanced computational procedures to derive a universal TB vaccine and one targeting East Africa. Our approach selects an optimal set of highly conserved, experimentally validated epitopes, with high projected population coverage (PPC). Through rigorous data analysis, five different potential vaccine combinations were selected each with PPC above 80% for East Africa and above 90% for the World. Two potential vaccines only contained CD8+ epitopes, while the others included both CD4+ and CD8+ epitopes. Our prime vaccine candidate was a putative seven-epitope ensemble comprising: SRGWSLIKSVRLGNA, KPRIITLTMNPALDI, AAHKGLMNIALAISA, FPAGGSTGSL, MLLAVTVSL, QSSFYSDW and KMRCGAPRY, with a 97.4% global PPC and a 92.7% East African PPC.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
BCG Vaccine chemistry
BCG Vaccine immunology
Computational Biology
Computer Simulation
Epitopes
Humans
Mycobacterium tuberculosis chemistry
Tuberculosis Vaccines chemistry
Tuberculosis Vaccines immunology
Tuberculosis Vaccines therapeutic use
Drug Design
Epitope Mapping methods
Mycobacterium tuberculosis immunology
Tuberculosis prevention & control
Tuberculosis Vaccines chemical synthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9142
- Volume :
- 97
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29567319
- Full Text :
- https://doi.org/10.1016/j.molimm.2018.03.007