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An analysis of the predictive value of the HIV Ag/Ab screening assay within the performance characteristics of the DiaSorin LIAISON XL for the detection of blood-borne viruses.
- Source :
-
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2018 May; Vol. 102, pp. 95-100. Date of Electronic Publication: 2018 Feb 27. - Publication Year :
- 2018
-
Abstract
- Background: Correct identification of blood borne viral infections, such as hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) is crucial in detection and follow up of infection in patients.<br />Objectives: We evaluated the diagnostic performance of the DiaSorin LIAISON XL (LIAISON XL) for screening of HBV, HCV and HIV infection. In addition, we investigated the variability of the signal-to-cuttoff ratio (S/CO) of the LIAISON XL HIV Ag/Ab assay and it's predictive value in subsequent confirmation of HIV-1 infection.<br />Study Design: We analyzed 16,497 blood samples on which HBV, HCV and HIV screening was performed. We defined A) archived samples previously tested with an arbitrary result in the Abbott ARCHITECT i2000SR system; B) prospectively collected samples which were simultaneously tested on the LIAISON XL and ARCHITECT i2000SR; C) prospectively collected serum samples for HIV testing which were tested solely on the LIAISON XL.<br />Results: The agreements of HBV-, HCV-, and HIV markers between the two compared systems are remarkably high. Among the samples which were prospectively tested for HIV Ab/Ag on the LIASON XL, 229 (1.6%) were reactive of which 141 (61.6%) could be confirmed. Increasing the signal-to-cutoff value to 4 could increase the positive predictive value (PPV) to 88.1% without decreasing sensitivity.<br />Conclusions: The LIAISON XL system proved to be an excellent system for diagnosing HBV, HCV, and HIV. Our data for the first time showed that increasing the HIV S/CO ratio was safe and increased the PPV for confirmed HIV infection in the tested population.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- HIV Antibodies blood
HIV Antigens blood
Hepatitis B Surface Antigens blood
Hepatitis C Antibodies blood
Humans
Prospective Studies
Retrospective Studies
Serologic Tests
Signal-To-Noise Ratio
HIV Infections diagnosis
Hepatitis B diagnosis
Hepatitis C diagnosis
Immunoassay standards
Mass Screening methods
Predictive Value of Tests
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5967
- Volume :
- 102
- Database :
- MEDLINE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 29547874
- Full Text :
- https://doi.org/10.1016/j.jcv.2018.02.018