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A Comprehensive Arabidopsis Yeast Two-Hybrid Library for Protein-Protein Interaction Studies: A Resource to the Plant Research Community.
- Source :
-
Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2018 Sep; Vol. 31 (9), pp. 899-902. Date of Electronic Publication: 2018 Jul 19. - Publication Year :
- 2018
-
Abstract
- Yeast-two-hybrid (Y2H) cDNA library screening is a valuable tool to uncover protein-protein interactions and represents a widely used method to investigate protein function. However, low transcript representation in cDNA libraries limits the depth of the screening. We have developed a Y2H library with cDNA made from Arabidopsis leaves exposed to several stressors as well as untreated leaves. The library was built using pooled mRNA extracted from plants challenged with plant and human bacterial pathogens, the flg22 elicitor, the phytotoxin coronatine, and several hormones associated with environmental stress responses. The purpose of such a library is to maximize the discovery of protein-protein interactions that occur under optimum conditions as well as during biotic and abiotic stresses.
- Subjects :
- Amino Acids metabolism
Arabidopsis physiology
Escherichia coli O157 physiology
Indenes metabolism
Plant Growth Regulators metabolism
Plant Leaves genetics
Plant Leaves physiology
Pseudomonas syringae physiology
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Salmonella typhimurium physiology
Stress, Physiological
Two-Hybrid System Techniques
Arabidopsis genetics
Gene Library
Protein Interaction Mapping methods
Subjects
Details
- Language :
- English
- ISSN :
- 0894-0282
- Volume :
- 31
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Publication Type :
- Academic Journal
- Accession number :
- 29547357
- Full Text :
- https://doi.org/10.1094/MPMI-02-18-0047-A