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How RNA transcripts coordinate DNA recombination and repair.
- Source :
-
Nature communications [Nat Commun] 2018 Mar 15; Vol. 9 (1), pp. 1091. Date of Electronic Publication: 2018 Mar 15. - Publication Year :
- 2018
-
Abstract
- Genetic studies in yeast indicate that RNA transcripts facilitate homology-directed DNA repair in a manner that is dependent on RAD52. The molecular basis for so-called RNA-DNA repair, however, remains unknown. Using reconstitution assays, we demonstrate that RAD52 directly cooperates with RNA as a sequence-directed ribonucleoprotein complex to promote two related modes of RNA-DNA repair. In a RNA-bridging mechanism, RAD52 assembles recombinant RNA-DNA hybrids that coordinate synapsis and ligation of homologous DNA breaks. In an RNA-templated mechanism, RAD52-mediated RNA-DNA hybrids enable reverse transcription-dependent RNA-to-DNA sequence transfer at DNA breaks that licenses subsequent DNA recombination. Notably, we show that both mechanisms of RNA-DNA repair are promoted by transcription of a homologous DNA template in trans. In summary, these data elucidate how RNA transcripts cooperate with RAD52 to coordinate homology-directed DNA recombination and repair in the absence of a DNA donor, and demonstrate a direct role for transcription in RNA-DNA repair.
- Subjects :
- DNA Breaks, Double-Stranded
DNA Repair genetics
DNA Repair physiology
RNA genetics
Rad52 DNA Repair and Recombination Protein genetics
Recombinational DNA Repair genetics
Recombinational DNA Repair physiology
Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
Rad52 DNA Repair and Recombination Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 29545568
- Full Text :
- https://doi.org/10.1038/s41467-018-03483-7