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Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease.
- Source :
-
The American journal of pathology [Am J Pathol] 2018 Jun; Vol. 188 (6), pp. 1345-1353. Date of Electronic Publication: 2018 Mar 12. - Publication Year :
- 2018
-
Abstract
- Trypanosoma cruzi infection in women of reproductive age is associated with congenital transmission and adverse pregnancy outcomes. The placenta is a key barrier to infection. Gene expression profiles of term placental environment from T. cruzi-seropositive (SP) and -seronegative (SN) mothers were characterized by RNA-Seq. Nine pools of placental RNA paired samples were used: three from SN and six from SP tissues. Each pool consisted of female/male newborns and vaginal/cesarean delivery binomials. No newborn was congenitally infected. T. cruzi satellite DNA quantitative PCR in placental tissues and maternal and neonatal blood, and parasite 18S quantitative RT-PCR from placental RNA were negative, except in three SP women's bloodstream. To identify pathways associated with maternal T. cruzi infection, a gene-set association analysis was implemented: SP placental samples showed overexpression of inflammatory response and lymphocytic activation, whereas numerous biosynthetic processes were down-regulated. About 42 genes showed a significant fold-change between SP and SN groups. KISS1 and CGB5 were down-regulated, whereas KIF12, HLA-G, PRG2, TAC3, FN1, and ATXN3L were up-regulated. Several expressed genes in SP placentas encode proteins associated with preeclampsia and miscarriage. This first transcriptomics study in human term placental environment shows a placental response that may affect the fetus while protecting it from parasite infection; this host response could be responsible for the low rate of congenital transmission in chronic Chagas disease.<br /> (Copyright © 2018 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adolescent
Adult
Chagas Disease complications
Chagas Disease parasitology
Female
Fetus parasitology
Gene Expression Profiling
Humans
Infant, Newborn
Male
Placenta parasitology
Pregnancy
Pregnancy Complications, Infectious parasitology
Young Adult
Chagas Disease genetics
Fetus metabolism
Gene Expression Regulation
Placenta metabolism
Pregnancy Complications, Infectious genetics
Trypanosoma cruzi genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1525-2191
- Volume :
- 188
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 29545200
- Full Text :
- https://doi.org/10.1016/j.ajpath.2018.02.011