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Alternative Carcinogenicity Screening Assay Using Colon Cancer Stem Cells: A Quantitative PCR (qPCR)-Based Prediction System for Colon Carcinogenesis.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2018 Apr 28; Vol. 28 (4), pp. 645-651. - Publication Year :
- 2018
-
Abstract
- The carcinogenicity of chemicals in the environment is a major concern. Recently, numerous studies have attempted to develop methods for predicting carcinogenicity, including rodent and cell-based approaches. However, rodent carcinogenicity tests for evaluating the carcinogenic potential of a chemical to humans are time-consuming and costly. This study focused on the development of an alternative method for predicting carcinogenicity using quantitative PCR (qPCR) and colon cancer stem cells. A toxicogenomic method, mRNA profiling, is useful for predicting carcinogenicity. Using microarray analysis, we optimized 16 predictive gene sets from five carcinogens (azoxymethane, 3,2'-dimethyl-4-aminobiphenyl, N -ethyl- n -nitrosourea, metronidazole, 4-( n -methyl- n -nitrosamino)-1-(3-pyridyl)-1-butanone) used to treat colon cancer stem cell samples. The 16 genes were evaluated by qPCR using 23 positive and negative carcinogens in colon cancer stem cells. Among them, six genes could differentiate between positive and negative carcinogens with a p -value of < or =0.05. Our qPCR-based prediction system for colon carcinogenesis using colon cancer stem cells is cost- and time-efficient. Thus, this qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.
- Subjects :
- Biomarkers, Tumor analysis
Biomarkers, Tumor genetics
Carcinogenicity Tests economics
Cell Survival drug effects
Colon
DNA Primers
Gene Expression Profiling methods
Gene Expression Regulation, Neoplastic genetics
Gene Regulatory Networks
HCT116 Cells
Humans
In Vitro Techniques
RNA, Messenger analysis
Real-Time Polymerase Chain Reaction economics
Time Factors
Carcinogenesis genetics
Carcinogenicity Tests methods
Carcinogens toxicity
Colonic Neoplasms chemically induced
Colonic Neoplasms genetics
Neoplastic Stem Cells
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1738-8872
- Volume :
- 28
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29539880
- Full Text :
- https://doi.org/10.4014/jmb.1712.12043