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Potential Point-of-Care Testing for Dengue Virus in the Field.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2018 Apr 25; Vol. 56 (5). Date of Electronic Publication: 2018 Apr 25 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- The four serotypes of dengue virus (DENV) cause one of the most important and rapidly emerging mosquito-borne viral diseases in humans. Of the currently available diagnostic tests for dengue, the reverse transcription-PCR (RT-PCR) assay is the most sensitive and specific, and so it is commonly used as the gold standard. However, the requirement of a sophisticated and expensive thermal cycler makes it very difficult to use as a point-of-care diagnostic test in resource-limited regions where dengue is endemic. Tsai et al. (J Clin Microbiol 56:e01865-17, 2018, https://doi.org/10.1128/JCM.01865-17) report the analytical and clinical performances of a reverse transcription-insulated isothermal PCR (RT-iiPCR) assay with a portable nucleic acid analyzer for rapid detection of the four DENV serotypes; its reproducibility and complete agreement on clinical samples with the multiplex RT-PCR assay developed by the Centers for Disease Control and Prevention suggest that the dengue RT-iiPCR is a potential point-of-care test. Compared with other DENV RNA detection methods, the unique isothermal PCR design of RT-iiPCR, together with further improvements, would represent a promising new type of field-deployable diagnostic test for dengue.<br /> (Copyright © 2018 American Society for Microbiology.)
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 56
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29514934
- Full Text :
- https://doi.org/10.1128/JCM.00203-18