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Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos.
- Source :
-
BMC developmental biology [BMC Dev Biol] 2018 Mar 05; Vol. 18 (1), pp. 5. Date of Electronic Publication: 2018 Mar 05. - Publication Year :
- 2018
-
Abstract
- Background: Tbx5 deficiency in zebrafish causes several abnormal phenotypes of the heart and pectoral fins. It has been reported that exogenous human growth hormone can enhance expression of downstream mediators in the growth hormone and insulin-like growth factor I (IGF-I) pathway and partially restore dysmorphogenesis in tbx5 morphants. This study aimed to further evaluate the effects of IGF-I on cell apoptosis and dysmorphogenesis in zebrafish embryos deficient for tbx5.<br />Results: Among the five studied groups of zebrafish embryos (wild-type embryos [WT], tbx5 morphants [MO], mismatched tbx5 morpholino-treated wild-type embryos [MIS], IGF-I-treated wild-type embryos [WTIGF1], and IGF-I-treated tbx5 morphants [MOIGF1]), the expression levels of the ifg1, igf1-ra, ifg-rb, erk1, and akt2 genes as well as the ERK and AKT proteins were significantly reduced in the MO group, but were partially restored in the MOIGF1 group. These expression levels remained normal in the WT, MIS, and WTIGF1 groups. Exogenous human IGF-I also reduced the incidence of phenotypic anomalies, decreased the expression levels of apoptotic genes and proteins, suppressed cell apoptosis, and improved survival of the MOIGF1 group.<br />Conclusions: These results suggest that IGF-I has an anti-apoptotic protective effect in zebrafish embryos with tbx5 deficiency.
- Subjects :
- Animals
Embryo, Nonmammalian drug effects
Gene Expression Regulation, Developmental drug effects
Humans
Morpholinos pharmacology
Myocardium metabolism
Myocytes, Cardiac drug effects
Myocytes, Cardiac metabolism
Phenotype
Phosphorylation drug effects
Survival Analysis
T-Box Domain Proteins metabolism
Zebrafish genetics
Apoptosis drug effects
Apoptosis genetics
Embryo, Nonmammalian metabolism
Insulin-Like Growth Factor I pharmacology
Morphogenesis drug effects
Morphogenesis genetics
T-Box Domain Proteins deficiency
Zebrafish embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-213X
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 29506474
- Full Text :
- https://doi.org/10.1186/s12861-017-0161-1