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Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes.
- Source :
-
The Journal of reproduction and development [J Reprod Dev] 2018 Apr 13; Vol. 64 (2), pp. 161-171. Date of Electronic Publication: 2018 Mar 02. - Publication Year :
- 2018
-
Abstract
- Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H <subscript>2</subscript> O <subscript>2</subscript> enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT.
- Subjects :
- 5-Methylcytosine analogs & derivatives
5-Methylcytosine metabolism
Active Transport, Cell Nucleus drug effects
Animals
Cell Nucleus drug effects
Cells, Cultured
Cumulus Cells cytology
Cumulus Cells drug effects
Cumulus Cells physiology
Female
Fertilization in Vitro
Hydrogen Peroxide toxicity
Male
Mice, Inbred ICR
Microscopy, Confocal
Oxidants toxicity
Oxidative Stress drug effects
Proteomics methods
Reactive Oxygen Species metabolism
Zygote cytology
Zygote drug effects
Zygote growth & development
Cell Nucleus enzymology
DNA Methylation drug effects
Ectogenesis drug effects
Epigenesis, Genetic drug effects
Peroxiredoxins metabolism
Zygote enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1348-4400
- Volume :
- 64
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 29503398
- Full Text :
- https://doi.org/10.1262/jrd.2018-005