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Genotoxicity evaluation of multi-component mixtures of polyaromatic hydrocarbons (PAHs), arsenic, cadmium, and lead using flow cytometry based micronucleus test in HepG2 cells.
- Source :
-
Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2018 Mar; Vol. 827, pp. 9-18. Date of Electronic Publication: 2018 Jan 10. - Publication Year :
- 2018
-
Abstract
- Some polyaromatic hydrocarbons (PAHs) and metals are known human carcinogens and the combined toxicity data of these co-contaminants are important for assessing their health risk. In this study, we have evaluated the combined genotoxicity, AhR activity and cell cycle parameters of four PAHs (benzo[a]pyrene (Ba]P), naphthalene (Nap), phenanthrene (Phe) and pyrene (Pyr)) and three metals (arsenic (As), cadmium (Cd), and lead (Pb)) in HepG2 cells using a flow cytometry based micronucleus (MN) test CAFLUX assay and nuclear fluorescence assay, respectively. The mixtures of B[a]P and metals induced a maximum of four fold increase in the MN formation compared to B[a]P alone. The higher combination of PAHs and metals did not significantly increase the MN formation. The mixtures of metals or non-carcinogenic PAHs were found to increase or decrease the aryl hydrocarbon receptor (AhR) activation of B[a]P in HepG2 cell based CAFLUX assay. Overall, the results showed that combined genotoxicity of PAHs and metals in HepG2 cells vary depending on the concentrations and number of the chemicals that are present in the mixtures and the effects of higher order combinations appear to be largely unpredictable from binary combinations. In this study, we have demonstrated the use of flow cytometry based MN test to screen the genotoxicity of environmental chemicals and its mixtures.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Carcinogens toxicity
Cell Survival
Hep G2 Cells
Humans
Mutagenicity Tests
Receptors, Aryl Hydrocarbon
Arsenic adverse effects
Cadmium adverse effects
DNA Damage
Flow Cytometry methods
Lead adverse effects
Micronuclei, Chromosome-Defective chemically induced
Micronucleus Tests methods
Polycyclic Aromatic Hydrocarbons adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3592
- Volume :
- 827
- Database :
- MEDLINE
- Journal :
- Mutation research. Genetic toxicology and environmental mutagenesis
- Publication Type :
- Academic Journal
- Accession number :
- 29502740
- Full Text :
- https://doi.org/10.1016/j.mrgentox.2018.01.002