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Antiviral effects and mechanisms of Yinhuapinggan granule against H1N1 influenza virus infection in RAW264.7 cells.
- Source :
-
Inflammopharmacology [Inflammopharmacology] 2018 Dec; Vol. 26 (6), pp. 1455-1467. Date of Electronic Publication: 2018 Mar 03. - Publication Year :
- 2018
-
Abstract
- Yinhuapinggan granule (YHPG), a modified prescription based on Ma-Huang-Tang (MHT), is used in traditional Chinese medicine (TCM) to treat influenza, cough, and viral pneumonia. In this study, we investigated the antiviral effects of YHPG by means of pre-, post-, and co-treatment, and its underlying mechanisms on regulating the levels of inflammatory-related cytokines, modulating the mRNA expressions of interferon-stimulated genes in influenza virus-infected murine macrophage cells (RAW264.7), and evaluating the protein expressions of key effectors in the Type I IFN and pattern recognition receptor (PRRs) signaling pathways. The results showed that YHPG markedly inhibited influenza virus (IFV) replication in pre-, post- and co-treatment assay, especially in post-treatment assay. Antiviral mechanisms studies revealed that YHPG (500 and 250 μg/mL) significantly up-regulated levels of IFN-β, IFN-stimulated genes (Mx-1, ISG-15 and ISG-56) compared with the IFV control group, while the levels of IL-6 and TNF-α were significantly down-regulated. Furthermore, western blot analysis results revealed that the protein expressions of the phosphorylated forms of TBK1, IRF3, ERK1/2, P38 MAPK and NF-κB p65 were significantly down-regulated in RAW264.7 cells with the YHPG (500 and 250 μg/mL) treatment, while the expression of the phosphorylated form of STAT1 was significantly enhanced. Based on these results, YHPG had antiviral effects in IFV-infected RAW264.7 cells, which might be associated with regulation of the inflammatory cytokines production, evaluation of the levels of IFN-stimulated genes, and modulation of the protein expressions of key effectors in the Type I IFN and PRRs signaling pathways.
- Subjects :
- Animals
Cell Survival drug effects
Cytokines metabolism
Gene Expression Regulation, Viral drug effects
Humans
Influenza, Human drug therapy
Influenza, Human virology
Interferons pharmacology
Mice
RAW 264.7 Cells
RNA, Viral antagonists & inhibitors
RNA, Viral biosynthesis
Signal Transduction drug effects
Virus Replication drug effects
Antiviral Agents pharmacology
Drugs, Chinese Herbal pharmacology
Influenza A Virus, H1N1 Subtype drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1568-5608
- Volume :
- 26
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Inflammopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 29502306
- Full Text :
- https://doi.org/10.1007/s10787-018-0457-1