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Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.
- Source :
-
Molecular microbiology [Mol Microbiol] 2018 May; Vol. 108 (4), pp. 379-396. Date of Electronic Publication: 2018 Mar 30. - Publication Year :
- 2018
-
Abstract
- Helicobacter pylori is anomalous among non nitrogen-fixing bacteria in containing an incomplete NIF system for Fe-S cluster assembly comprising two essential proteins, NifS (cysteine desulfurase) and NifU (scaffold protein). Although nifU deletion strains cannot be obtained via the conventional gene replacement, a NifU-depleted strain was constructed and shown to be more sensitive to oxidative stress compared to wild-type (WT) strains. The hp1492 gene, encoding a putative Nfu-type Fe-S cluster carrier protein, was disrupted in three different H. pylori strains, indicating that it is not essential. However, Δnfu strains have growth deficiency, are more sensitive to oxidative stress and are unable to colonize mouse stomachs. Moreover, Δnfu strains have lower aconitase activity but higher hydrogenase activity than the WT. Recombinant Nfu was found to bind either one [2Fe-2S] or [4Fe-4S] cluster/dimer, based on analytical, UV-visible absorption/CD and resonance Raman studies. A bacterial two-hybrid system was used to ascertain interactions between Nfu, NifS, NifU and each of 36 putative Fe-S-containing target proteins. Nfu, NifS and NifU were found to interact with 15, 6 and 29 putative Fe-S proteins respectively. The results indicate that Nfu, NifS and NifU play a major role in the biosynthesis and/or delivery of Fe-S clusters in H. pylori.<br /> (© 2018 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Bacterial Proteins genetics
Bacterial Proteins metabolism
Female
Humans
Iron-Sulfur Proteins genetics
Mice
Mice, Inbred C57BL
Oxidative Stress genetics
Recombinant Proteins genetics
Recombinant Proteins metabolism
Amino Acid Sequence
Base Sequence
Helicobacter pylori genetics
Iron-Sulfur Proteins metabolism
Sequence Deletion
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2958
- Volume :
- 108
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29498770
- Full Text :
- https://doi.org/10.1111/mmi.13942