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Anti-Inflammatory and Anti-Apoptotic Effects of Acer Palmatum Thumb. Extract, KIOM-2015EW, in a Hyperosmolar-Stress-Induced In Vitro Dry Eye Model.
- Source :
-
Nutrients [Nutrients] 2018 Feb 28; Vol. 10 (3). Date of Electronic Publication: 2018 Feb 28. - Publication Year :
- 2018
-
Abstract
- The aim of this study was to assess the anti-inflammatory and anti-apoptotic effects of KIOM-2015EW, the hot-water extract of maple leaves in hyperosmolar stress (HOS)-induced human corneal epithelial cells (HCECs). HCECs were exposed to hyperosmolar medium and exposed to KIOM-2015EW with or without the hyperosmolar media. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production and apoptosis were observed, and the activation of mitogen-activated protein kinases (MAPKs) including extracellular signal regulated kinase (ERK), p38 and c-JUN N-terminal kinase (JNK) signaling and nuclear factor (NF)-κB was confirmed. Compared to isomolar medium, the induction of cell cytotoxicity significantly increased in HCECs exposed to hyperosmolar medium in a time-dependent manner. KIOM-2015EW-treatment significantly reduced the mRNA and protein expression of pro-inflammatory mediators and apoptosis. KIOM-2015EW-treatment inhibited HOS-induced MAPK signaling activation. Additionally, the HOS-induced increase in NF-κB phosphorylation was attenuated by KIOM-2015EW. The results demonstrated that KIOM-2015EW protects the ocular surface by suppressing inflammation in dry eye disease, and suggest that KIOM-2015EW may be used to treat several ocular surface diseases where inflammation plays a key role.<br />Competing Interests: The authors declare no competing financial interests.
- Subjects :
- Anti-Inflammatory Agents isolation & purification
Cells, Cultured
Dose-Response Relationship, Drug
Epithelium, Corneal metabolism
Epithelium, Corneal pathology
Humans
Inflammation Mediators metabolism
Interleukin-1beta metabolism
Interleukin-6 metabolism
Mitogen-Activated Protein Kinases metabolism
Phosphorylation
Phytotherapy
Plant Extracts isolation & purification
Plant Leaves
Plants, Medicinal
Signal Transduction drug effects
Time Factors
Transcription Factor RelA metabolism
Tumor Necrosis Factor-alpha metabolism
Xerophthalmia etiology
Xerophthalmia metabolism
Xerophthalmia pathology
Acer chemistry
Anti-Inflammatory Agents pharmacology
Apoptosis drug effects
Epithelium, Corneal drug effects
Osmotic Pressure
Plant Extracts pharmacology
Xerophthalmia prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6643
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nutrients
- Publication Type :
- Academic Journal
- Accession number :
- 29495608
- Full Text :
- https://doi.org/10.3390/nu10030282