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The Aquaporin 1 Inhibitor Bacopaside II Reduces Endothelial Cell Migration and Tubulogenesis and Induces Apoptosis.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2018 Feb 26; Vol. 19 (3). Date of Electronic Publication: 2018 Feb 26. - Publication Year :
- 2018
-
Abstract
- Expression of aquaporin-1 (AQP1) in endothelial cells is critical for their migration and angiogenesis in cancer. We tested the AQP1 inhibitor, bacopaside II, derived from medicinal plant Bacopa monnieri , on endothelial cell migration and tube-formation in vitro using mouse endothelial cell lines (2H11 and 3B11) and human umbilical vein endothelial cells (HUVEC). The effect of bacopaside II on viability, apoptosis, migration and tubulogenesis was assessed by a proliferation assay, annexin-V/propidium iodide flow cytometry, the scratch wound assay and endothelial tube-formation, respectively. Cell viability was reduced significantly for 2H11 at 15 μM ( p = 0.037), 3B11 at 12.5 μM ( p = 0.017) and HUVEC at 10 μM ( p < 0.0001). At 15 μM, the reduced viability was accompanied by an increase in apoptosis of 38%, 50% and 32% for 2H11, 3B11 and HUVEC, respectively. Bacopaside II at ≥10 μM significantly reduced migration of 2H11 ( p = 0.0002) and 3B11 ( p = 0.034). HUVECs were most sensitive with a significant reduction at ≥7.5 μM ( p = 0.037). Tube-formation was reduced with a 15 μM dose for all cell lines and 10 μM for 3B11 ( p < 0.0001). These results suggest that bacopaside II is a potential anti-angiogenic agent.<br />Competing Interests: The authors declare no conflict of interest. The funding sponsors had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript; nor in the decision to publish the results.
- Subjects :
- Cell Movement drug effects
Cell Proliferation drug effects
Cell Survival drug effects
Dose-Response Relationship, Drug
Human Umbilical Vein Endothelial Cells
Humans
Neovascularization, Physiologic drug effects
Apoptosis drug effects
Aquaporin 1 antagonists & inhibitors
Endothelial Cells drug effects
Endothelial Cells metabolism
Saponins pharmacology
Triterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 19
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29495367
- Full Text :
- https://doi.org/10.3390/ijms19030653