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Assessment of HER2 status in breast cancer biopsies is not affected by accelerated tissue processing.
- Source :
-
Histopathology [Histopathology] 2018 Jul; Vol. 73 (1), pp. 81-89. Date of Electronic Publication: 2018 Apr 24. - Publication Year :
- 2018
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Abstract
- Aims: To establish whether core needle biopsy (CNB) specimens processed with an accelerated processing method with short fixation time can be used to determine accurately the human epidermal growth factor receptor 2 (HER2) status of breast cancer.<br />Methods and Results: A consecutive case-series from two high-volume breast clinics was created. We compared routine HER2 immunohistochemistry (IHC) assessment between accelerated processing CNB specimens and routinely processed postoperative excision specimens. Additional amplification-based testing was performed in cases with equivocal results. The formalin fixation time was less than 2 h and between 6 and 72 h, respectively. Fluorescence in-situ hybridisation and multiplex ligation-dependent probe amplification were used for amplification testing. One hundred and forty-four cases were included, 15 of which were HER2-positive on the routinely processed excision specimens. On the CNB specimens, 44 were equivocal on IHC and required an amplification-based test. Correlation between the CNB specimens and the corresponding excision specimens was high for final HER2 status, with an accuracy of 97% and a kappa of 0.85.<br />Conclusions: HER2 status can be determined reliably on CNB specimens with accelerated processing time using standard clinical testing methods. Using this accelerated technology the minimum 6 h of formalin fixation, which current guidelines consider necessary, can be decreased safely. This allows for a complete and expedited histology-based diagnosis of breast lesions in the setting of a one-stop-shop, same-day breast clinic.<br /> (© 2018 The Authors. Histopathology Published by John Wiley & Sons Ltd.)
Details
- Language :
- English
- ISSN :
- 1365-2559
- Volume :
- 73
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 29495112
- Full Text :
- https://doi.org/10.1111/his.13507