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Potential added value of a RT-qPCR method of SOX 11 expression, in the context of a multidisciplinary diagnostic assessment of B cell malignancies.
- Source :
-
Experimental hematology & oncology [Exp Hematol Oncol] 2018 Feb 20; Vol. 7, pp. 5. Date of Electronic Publication: 2018 Feb 20 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Background: Expression of SRY [sex-determining region Y]-box11 (SOX11) is specific to mantle cell lymphoma (MCL) and contributes, in conjunction with immunoglobulin variable heavy chain gene mutation status, to the identification of two forms of this disease.<br />Methods: The aim of this report was firstly, to design an easy and suitable RT-qPCR method to quantify SOX11 mRNA expression in mantle cell lymphoma and other B cell malignancies with the proper reference gene; secondly, to define the best threshold of relative quantity of SOX11 mRNA in order to reach the best compromise between sensitivity and specificity.<br />Results: For best discrimination of MCL and non-MCL groups we determined an area under the curve (AUC) of 0.9750 and a threshold of 1.76 with 100% sensitivity and 88% specificity. AUC and threshold values of respectively 0.91/1.346 [87% sensitivity, 80% specificity] and 0.9525/1.7120 [100% sensitivity, 88% specificity] for GAPDH and RPLP0 respectively denote that the RPLP0 reference gene alone is sufficient for PCR housekeeping gene.<br />Conclusion: This work describes an RT-qPCR assay for SOX11 expression in order to better characterize MCL at diagnosis. Further studies on larger cohorts are needed to evaluate this molecular tool, especially for the follow-up of minimal residual disease.
Details
- Language :
- English
- ISSN :
- 2162-3619
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Experimental hematology & oncology
- Publication Type :
- Academic Journal
- Accession number :
- 29484276
- Full Text :
- https://doi.org/10.1186/s40164-018-0097-6