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A method for red blood cell biotinylation in a closed system.
- Source :
-
Transfusion [Transfusion] 2018 Apr; Vol. 58 (4), pp. 896-904. Date of Electronic Publication: 2018 Feb 15. - Publication Year :
- 2018
-
Abstract
- Background: Several circumstances require the accurate measurement of red blood cell (RBC) survival and clearance, such as determination of posttransfusion recovery of stored RBCs to investigate the effect of new additive solutions. To this end, biotin as a marker of RBCs to track donor RBCs in the blood of the recipient has been used in many studies. However, so far only experimental, nonvalidated, biotin-labeled red cell concentrates (RCCs) are transfused. The goal of this study was to produce a standardized biotin-labeled RCC product in a fast, simple, and sterile manner that can be used for clinical research and for the evaluation of new blood products according to Good Practice Guidelines (GPG) for blood establishments.<br />Study Design and Methods: RCC fractions were labeled with two different concentrations of biotinylation reagent in a closed system, to prevent bacterial contamination of the end product. Using flow cytometry, the reproducibility and robustness of the biotin labeling was assessed, as well as the stability of the biotin label on the (un-)irradiated RCC fraction. Additionally, parameters such as phosphatidylserine (PS) exposure, sodium (Na), potassium (K), free hemoglobin, adenosine triphosphate (ATP), pH, and morphology were determined prior to and after biotin labeling to rule out detrimental effects of the labeling procedure on the RCC.<br />Results: Our data show that RCCs can be labeled under sterile conditions in a closed system with two different biotinylation reagent concentrations, without affecting the biological activity.<br />Conclusion: An easy, rapid (<2 hr), and robust method was developed to manufacture biotin-labeled RCCs for clinical research compliant to GPG.<br /> (© 2018 AABB.)
- Subjects :
- Adenine
Adenosine Triphosphate blood
Annexin A5 blood
Bacteremia prevention & control
Biotin pharmacology
Blood Preservation
Cell Shape
Cell Survival
Glucose
Hemoglobins analysis
Humans
Hydrogen-Ion Concentration
Indicators and Reagents
Mannitol
Phosphatidylserines blood
Potassium blood
Sodium blood
Sodium Chloride
Biotinylation methods
Cell Tracking methods
Erythrocyte Transfusion
Erythrocytes chemistry
Erythrocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1537-2995
- Volume :
- 58
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 29446461
- Full Text :
- https://doi.org/10.1111/trf.14535