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Recombinant ApxIV protein enhances protective efficacy against Actinobacillus pleuropneumoniae in mice and pigs.
- Source :
-
Journal of applied microbiology [J Appl Microbiol] 2018 Jun; Vol. 124 (6), pp. 1366-1376. Date of Electronic Publication: 2018 Mar 13. - Publication Year :
- 2018
-
Abstract
- Aims: Available bacterins, commercial or autogenous, for Actinobacillus pleuropneumoniae disease control have, thus far, shown debatable protective efficacy and only in homologous challenges. Our study sought to determine whether the addition of reombinant protein ApxIV to the multicomponent vaccine could enhance protection against homologous and heterologous challenge of A. pleuropneumoniae.<br />Methods and Results: The virulence of ApxI, ApxII, ApxIV and OMP were cloned and expressed using a prokaryotic system; these recombinant proteins were combined with inactivated A. pleuropneumoniae serovar 1 to formulate different multicomponent vaccines. Immune response and protective efficacy of the vaccines were evaluated in mice and pigs. A protection rate of 67% was observed against heterologous challenge in mice vaccinated with the rApxIV formulation. Piglets vaccinated with vaccine containing ApxIV produced significantly higher antibody titre and provided complete protection and reduced gross lesions by 67% when compared with the nonimmunized group after homologous challenge. Additionally, flow cytometry analysis showed significant cellular immune response.<br />Conclusions: The results of our vaccination experiments revealed that a combination of inactivated bacteria and the recombinant antigens rApxI, rApxII, rApxIV and rOMP can provide effective protection against heterologous A. pleuropneumoniae challenge.<br />Significance and Impact of the Study: The addition of ApxIV to the multicomponent vaccine could enhance homologous and heterologous protection in mice and pigs, respectively, against challenge by A. pleuropneumoniae.<br /> (© 2018 The Society for Applied Microbiology.)
- Subjects :
- Actinobacillus Infections immunology
Actinobacillus Infections microbiology
Actinobacillus Infections prevention & control
Actinobacillus pleuropneumoniae genetics
Animals
Antibodies, Bacterial immunology
Bacterial Proteins administration & dosage
Bacterial Proteins genetics
Bacterial Vaccines administration & dosage
Bacterial Vaccines genetics
Bacterial Vaccines immunology
Female
Mice
Recombinant Proteins administration & dosage
Recombinant Proteins genetics
Recombinant Proteins immunology
Swine
Swine Diseases immunology
Swine Diseases microbiology
Vaccination
Actinobacillus Infections veterinary
Actinobacillus pleuropneumoniae immunology
Bacterial Proteins immunology
Swine Diseases prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2672
- Volume :
- 124
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29431246
- Full Text :
- https://doi.org/10.1111/jam.13726