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"NOTA-PRGD 2 and NODAGA-PRGD 2 : Bioconjugation, characterization, radiolabelling, and design space".
- Source :
-
Journal of labelled compounds & radiopharmaceuticals [J Labelled Comp Radiopharm] 2018 May 30; Vol. 61 (6), pp. 487-500. Date of Electronic Publication: 2018 Apr 25. - Publication Year :
- 2018
-
Abstract
- This work reports on the development of amide bond bioconjugation for the production of -NOTA and -NODAGA PRGD <subscript>2</subscript> using batch strategy and microfluidic reactor technology. The final radiolabelling step was fully optimized using Design of Experiments and Design Space approaches, hence targeting robust labelling yields in routine. Optimal labelling conditions were defined in sodium acetate buffer as 168 μg/mL peptide concentration, 4.9 pH, 47.5°C temperature, and 12.5-minute reaction time. Upon optimization, the Gallium-68 radiolabelling was fully automated. All the work was designed to be compliant to the GMP environment and to support the pharmaceutical scale-up.<br /> (Copyright © 2018 John Wiley & Sons, Ltd.)
- Subjects :
- Amides chemistry
Automation instrumentation
Automation methods
Chemistry Techniques, Synthetic instrumentation
Chemistry Techniques, Synthetic methods
Microfluidics instrumentation
Microfluidics methods
Polycyclic Compounds chemistry
Amides chemical synthesis
Gallium Radioisotopes chemistry
Oligopeptides chemistry
Organometallic Compounds chemistry
Polycyclic Compounds chemical synthesis
Radiopharmaceuticals chemical synthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1099-1344
- Volume :
- 61
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of labelled compounds & radiopharmaceuticals
- Publication Type :
- Academic Journal
- Accession number :
- 29430693
- Full Text :
- https://doi.org/10.1002/jlcr.3613