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Generation of genipin cross-linked fibrin-agarose hydrogel tissue-like models for tissue engineering applications.

Authors :
Campos F
Bonhome-Espinosa AB
Vizcaino G
Rodriguez IA
Duran-Herrera D
López-López MT
Sánchez-Montesinos I
Alaminos M
Sánchez-Quevedo MC
Carriel V
Source :
Biomedical materials (Bristol, England) [Biomed Mater] 2018 Feb 08; Vol. 13 (2), pp. 025021. Date of Electronic Publication: 2018 Feb 08.
Publication Year :
2018

Abstract

The generation of biomimetic and biocompatible artificial tissues is the basic research objective for tissue engineering (TE). In this sense, the biofabrication of scaffolds that resemble the tissues' extracellular matrix is an essential aim in this field. Uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH, respectively) have emerged as promising scaffolds in TE, but their structure and biomechanical properties must be improved in order to broaden their TE applications. Here, we generated and characterized novel membrane-like models with increased structural and biomechanical properties based on the chemical cross-linking of FAH and NFAH with genipin (GP at 0.1%, 0.25%, 0.5% and 0.75%). Furthermore, the scaffolds were subjected to rheological (G, G', G″ modulus), ultrastructural and ex vivo biocompatibility analyses. Results showed that all GP concentrations increased the stiffness (G) and especially the elasticity (G') of FAH and NFAH. Ultrastructural analyses demonstrated that GP and nanostructuration of FAH allowed us to control the porosity of FAH. In addition, biological studies revealed that higher concentration of GP (0.75%) started to compromise the cell function and viability. Finally, this study demonstrated the possibility to generate natural and biocompatible FAH and NFAH with improved structural and biomechanical properties by using 0.1%-0.5% of GP. However, further in vivo studies are needed in order to demonstrate the biocompatibility, biodegradability and regeneration capability of these cross-linked scaffolds.

Details

Language :
English
ISSN :
1748-605X
Volume :
13
Issue :
2
Database :
MEDLINE
Journal :
Biomedical materials (Bristol, England)
Publication Type :
Academic Journal
Accession number :
29420310
Full Text :
https://doi.org/10.1088/1748-605X/aa9ad2