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In Vitro Anti/Pro-oxidant Activities of R. ferruginea Extract and Its Effect on Glioma Cell Viability: Correlation with Phenolic Compound Content and Effects on Membrane Dynamics.
- Source :
-
The Journal of membrane biology [J Membr Biol] 2018 Apr; Vol. 251 (2), pp. 247-261. Date of Electronic Publication: 2018 Feb 08. - Publication Year :
- 2018
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Abstract
- Rapanea ferruginea antioxidant and antitumoral properties were not explored before in literature. This study aimed to investigate these biological activities for the R. ferruginea leaf extract and correlate them with its phenolic content and influence in biological membrane dynamics. Thus, in this study, anti/pro-oxidative properties of R. ferruginea leaf extract by in vitro DPPH and TBARS assays, with respect to the free radical reducing potential and to its activity regarding membrane free radical-induced peroxidation, respectively. Furthermore, preliminary tests related to the extract effect on in vitro glioma cell viability were also performed. In parallel, the phenolic content was detected by HPLC-DAD and included syringic and trans-cinnamic acids, quercetrin, catechin, quercetin, and gallic acid. In an attempt to correlate the biological activity of R. ferruginea extract and its effect on membrane dynamics, the molecular interaction between the extract and a liposomal model with natural-sourced phospholipids was investigated. Location and changes in vibrational, rotational, and translational lipid motions, as well as in the phase state of liposomes, induced by R. ferruginea extract, were monitored by Fourier-transform infrared spectroscopy, nuclear magnetic resonance, differential scanning calorimetry, and UV-visible spectroscopy. In its free form, the extract showed promising in vitro antioxidant properties. Free-form extract (at 1000µ g/mL) exposure reduced glioma cell in vitro viability in 40%, as evidenced by MTT tests. Pro-oxidant behavior was observed when the extract was loaded into liposomes. A 70.8% cell viability reduction was achieved with 500 µg/mL of liposome-loaded extract. The compounds of R. ferruginea extract ordered liposome interface and disorder edits a polar region. Phenolic content, as well as membrane interaction and modulation may have an important role in the oxidative and antitumoral activities of the R. ferruginea leaf extract.
- Subjects :
- Antioxidants metabolism
Catechin pharmacology
Cell Line, Tumor
Gallic Acid pharmacology
Glioma metabolism
Humans
Liposomes chemistry
Oxidation-Reduction drug effects
Phenol chemistry
Quercetin analogs & derivatives
Quercetin pharmacology
Reactive Oxygen Species metabolism
Spectroscopy, Fourier Transform Infrared
Cell Survival drug effects
Myrsine chemistry
Plant Extracts chemistry
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1424
- Volume :
- 251
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of membrane biology
- Publication Type :
- Academic Journal
- Accession number :
- 29417170
- Full Text :
- https://doi.org/10.1007/s00232-018-0017-z