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Development and validation of analytical method for the estimation of nateglinide in rabbit plasma.
- Source :
-
Journal of pharmaceutical analysis [J Pharm Anal] 2012 Dec; Vol. 2 (6), pp. 492-498. Date of Electronic Publication: 2012 May 18. - Publication Year :
- 2012
-
Abstract
- Nateglinide has been widely used in the treatment of type-2 diabetics as an insulin secretogoga. A reliable, rapid, simple and sensitive reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of nateglinide in rabbit plasma. The method was developed on Hypersil BDSC-18 column (250 mm×4.6 mm, 5 mm) using a mobile phase of 10 mM phosphate buffer (pH 2.5) and acetonitrile (35:65, v/v). The elute was monitored with the UV-vis detector at 210 nm with a flow rate of 1 mL/min. Calibration curve was linear over the concentration range of 25-2000 ng/mL. The retention times of nateglinide and internal standard (gliclazide) were 9.608 min and 11.821 min respectively. The developed RP-HPLC method can be successfully applied to the quantitative pharmacokinetic parameters determination of nateglinide in rabbit model.
Details
- Language :
- English
- ISSN :
- 2095-1779
- Volume :
- 2
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 29403789
- Full Text :
- https://doi.org/10.1016/j.jpha.2012.05.002